Lysostaphin is a staphylolytic protein of growing interest from biotechnological and pharmaceutical industry due to its potential use in preventing and combating staphylococcal infections. Here, we describe an optimized method for production of lysostaphin in an inductionless system utilizing constitutive promoter from staphylococcal toxin-antitoxin system PemIK-Sa1. We investigated the influence of ribosome-binding site sequence, Escherichia coli producer strain and growth media on yield and kinetics of recombinant protein production. Lysostaphin was purified in its native active form using one-step cation-exchange chromatography. The system provides a method for cost-efficient and scalable protein production, and can be applied to produce other biotechnologically significant proteins.

中文翻译：

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利用毒素-抗毒素系统的启动子通过非专有方法生产溶葡萄球菌素。

溶葡萄球菌素是一种在生物技术和制药工业中日益受到关注的葡萄球菌溶蛋白，因为它在预防和对抗葡萄球菌感染中具有潜在的用途。在这里，我们描述了一种优化的方法，用于在无诱导系统中利用葡萄球菌毒素-抗毒素系统PemIK-Sa1的组成型启动子生产溶葡萄球菌素。我们调查了核糖体结合位点序列，大肠杆菌生产菌株和生长培养基对重组蛋白生产的产量和动力学的影响。溶葡萄球菌素使用一步阳离子交换色谱法以其天然活性形式纯化。该系统提供了一种经济高效且可扩展的蛋白质生产方法，可用于生产其他具有生物技术意义的蛋白质。