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Qualification of a surface plasmon resonance assay to determine binding of IgG-type antibodies to complement component C1q.
Biologicals ( IF 1.7 ) Pub Date : 2019-08-21 , DOI: 10.1016/j.biologicals.2019.08.004
Milan Jovic 1 , Florian Cymer 1
Affiliation  

Interaction of therapeutic antibodies with complement component C1q are frequently part of pharmaceutical characterization and production process comparability studies. Assays currently used to assess this interaction and/or the activation of the complement cascade are often cumbersome, time consuming or imprecise. We here report the further development, successful qualification and suitability evaluation of an SPR-based C1q binding assay for the characterization of IgG-C1q interactions. We evaluate different IgG subtypes and well-described mutants of IgG1. The assay offers a suitable alternative for extended characterization of interactions of IgG1 and IgG3 with complement component C1q.



中文翻译:

表面等离振子共振测定法的资格,以确定IgG型抗体与补体成分C1q的结合。

治疗性抗体与补体成分C1q的相互作用通常是药物表征和生产过程可比性研究的一部分。当前用于评估这种相互作用和/或补体级联的激活的测定通常是麻烦,费时或不精确的。我们在此报告了基于SPR的C1q结合测定用于IgG-C1q相互作用表征的进一步发展,成功的鉴定和适用性评估。我们评估不同的IgG亚型和IgG1的突变体。该测定法为扩展表征IgG1和IgG3与补体成分C1q相互作用提供了合适的替代方法。

更新日期:2019-08-21
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