Ligand regulation of the binding of [35S]GTPgammaS is one of the most widely used methods to measure receptor activation of heterotrimeric G proteins. However, until recently this method was largely restricted to receptors that interact with members of the family of pertussis-toxin-sensitive G proteins. Here, the reasons for this restriction are discussed and recent approaches that have extended the utility of this method such that it is now suitable for analysis of the activation of any heterotrimeric G protein are reviewed.

中文翻译：

---

原理：扩展[35S] GTPγS结合测定法的实用性。

[35S]GTPγS的结合的配体调节是测量异源三聚体G蛋白受体激活的最广泛使用的方法之一。然而，直到最近，该方法仍主要限于与百日咳毒素敏感的G蛋白家族成员相互作用的受体。在此，讨论了这种限制的原因，并综述了扩展该方法实用性的最新方法，以使其现在适用于分析任何异源三聚体G蛋白的活化。