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A 3D construct of the intestinal canal with wrinkle morphology on a centrifugation configuring microfluidic chip.
Biofabrication ( IF 9 ) Pub Date : 2019-07-01 , DOI: 10.1088/1758-5090/ab21b0
Yu Wang 1 , Zixing Shao , Wenchen Zheng , Yuanyuan Xie , Guoan Luo , Mingyu Ding , Qionglin Liang
Affiliation  

A new in vitro gut microfluidic chip that mimics in vivo intestinal canal morphology and stimulation is developed to contribute to research into tissue engineering, and intestinal development and function. This strategy utilizes centrifugation to configure spatial cells along the side wall of a vertical cylinder-like microfluidic chamber, by which a tubular intestinal epithelium cell sheet is formed. Diverse intestinal cell lines are inoculated to address this approach. Furthermore, to generate microenvironmental stimulation, low-level centrifugation introduces fluid flow to this microfluidic system perpendicularly acting on cell sheet cultivation for several days. Fluid flow engenders the sectional cell sheet to bend toward the cell chamber lumen, which manifests an intestinal epithelium vaulted and wrinkle morphology. This may mimic the fluid flow existing in in vivo material transportation and the absorption of the gut epithelium barrier. In addition, the same fluid flow stimulation was reproduced in another Transwell system, which also exhibited a wrinkle epithelium cell sheet. Under fluid flow stimulation, some of the villus specific genes' expression level increased in the microfluidics and Transwell insert. Thus, this new centrifugation configuring gut microfluidic chip may offer novel insights into the research of intestinal structure and function.

中文翻译:

在离心配置微流控芯片上具有皱纹形态的肠道的3D构建。

一种新的模仿肠道小肠形态和刺激的体外肠道微流控芯片被开发出来,以促进对组织工程,肠道发育和功能的研究。该策略利用离心作用沿垂直的圆柱状微流腔室的侧壁配置空间细胞,通过该空间细胞形成管状肠上皮细胞片。接种了多种肠细胞系以解决该方法。此外,为了产生微环境刺激,低水平离心将流体流引入到垂直作用于细胞片培养几天的该微流体系统。流体流动使切片的细胞片向细胞腔内腔弯曲,这表现出肠上皮弯曲和皱纹的形态。这可以模拟体内物质运输中存在的流体流动以及肠上皮屏障的吸收。另外,在另一个Transwell系统中也再现了相同的流体流动刺激,该系统还表现出皱纹上皮细胞片。在流体流动刺激下,微流体和Transwell插入物中某些绒毛特定基因的表达水平增加。因此,这种新型的离心配置肠道微流控芯片可以为肠道结构和功能的研究提供新的见解。微流体和Transwell插入物中的表达水平增加。因此,这种新型的离心配置肠道微流控芯片可以为肠道结构和功能的研究提供新的见解。微流体和Transwell插入物中的表达水平增加。因此,这种新型的离心配置肠道微流控芯片可以为肠道结构和功能的研究提供新的见解。
更新日期:2019-11-01
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