Studies have been conducted on the pathogenicity of periodontopathogens in cultures of endothelial cells on two‐dimensional (2D) polystyrene surfaces, where the monolayer formed is not exposed to proteins of the subendothelial matrix. In this work, we developed a culture system by seeding human coronary artery endothelial cells (HCAECs) onto three‐dimensional (3D) scaffolds of collagen type I, a subendothelial protein. The inflammatory responses of the HCAEC monolayers, formed either on 3D scaffolds or directly on a 2D polystyrene plate, to lipopolysaccharide (LPS) from Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) were evaluated. The transcription of 3 genes, the secretion of 40 cytokines and 2 prostanoids, and the adhesion of monocytes to 2D and 3D cultures with or without exposure to lipopolysaccharides (control) were assessed. HCAECs exhibited differences in transcriptional and secretory profiles between the 3D and 2D models. In addition, the inflammatory responses of HCAEC to Aa‐LPS and Pg‐LPS differed between the two models. In 3D cultures treated with Aa‐LPS, the levels of IL‐8, RANTES, G‐CSF, ICAM‐1, IL‐6, and TXA2 were significantly higher than those in the controls. In 2D cultures treated with Aa‐LPS, IL‐8, RANTES, G‐CSF, ICAM‐1, TNF‐RI, PGI2, and TXA2 levels were significantly higher than those in their controls. In the presence of Aa‐LPS, monocyte adhesion did not differ between treated and control 3D cultures but was significantly higher in treated 2D cultures than in the controls. In response to Pg‐LPS, cytokine‐prostaglandin secretion and monocyte adhesion did not differ between 3D and 2D cultures. These data indicate that HCAECs respond differently to these two types of LPS.

中文翻译：

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三维支架上的内皮细胞对牙周病原体脂多糖的不同反应。

已经对二维（2D）聚苯乙烯表面上内皮细胞培养物中牙周病原体的致病性进行了研究，其中形成的单层未暴露于内皮下基质的蛋白质。在这项工作中，我们通过将人冠状动脉内皮细胞（HCAEC）播种到I型胶原（一种内皮下蛋白）的三维（3D）支架上，从而开发了一种培养系统。在3D支架上或直接在2D聚苯乙烯板上形成的HCAEC单层对来自集热放线杆菌（Aa）和牙龈卟啉单胞菌（Pg）的脂多糖（LPS）的炎症反应）进行了评估。评估了3个基因的转录，40种细胞因子和2种类前列腺素的分泌以及单核细胞在暴露于或未暴露于脂多糖（对照）的情况下对2D和3D培养物的粘附。HCAEC在3D和2D模型之间表现出转录和分泌特征的差异。此外，两种模型之间HCAEC对Aa-LPS和Pg-LPS的炎症反应也不同。在用Aa-LPS处理的3D培养物中，IL-8，RANTES，G-CSF，ICAM-1，IL-6和TXA2的水平显着高于对照组。在用Aa-LPS处理的二维培养物中，IL-8，RANTES，G-CSF，ICAM-1，TNF-RI，PGI2和TXA2的水平明显高于对照组。在Aa‐LPS的情况下，在处理过的3D培养物和对照3D培养物之间单核细胞粘附没有差异，但是在处理过的2D培养物中单核细胞粘附力显着高于对照。在响应Pg-LPS时，3D和2D培养物之间的细胞因子-前列腺素分泌和单核细胞粘附没有差异。这些数据表明，HCAEC对这两种LPS的反应不同。