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Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma.
BMC Molecular Biology ( IF 4.619 ) Pub Date : 2019-01-03 , DOI: 10.1186/s12867-018-0119-2
Maciej Szmidt 1 , Adrian Stankiewicz 2 , Kaja Urbańska 1 , Sławomir Jaworski 3 , Marta Kutwin 3 , Mateusz Wierzbicki 3 , Marta Grodzik 3 , Beata Burzyńska 4 , Monika Góra 4 , André Chwalibog 5 , Ewa Sawosz 3
Affiliation  

Recently different forms of nanographene were proposed as the material with high anticancer potential. However, the mechanism of the suppressive activity of the graphene on cancer development remains unclear. We examined the effect of oxygenated, reduced and pristine graphene on the gene expression in glioblastoma U87 cell line. Conducting microarrays and RT-qPCR analysis we explored that graphene oxide (rather than reduced graphene oxide and pristine graphene) down-regulates the mRNA expression of mitochondrial oxidative phosphorylation (OXPHOS) nuclear genes of complexes I, III, IV and V. The presented results provide first evidence for the hypothesis that the suppressed growth of GBM can be the consequence of down-regulation of OXPHOS protein expression and decreased ATP level. We suggest that changes in the expression of OXPHOS genes identified in our study may mediate the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells. However, further investigations with different cell lines, regarding expression, regulation and activity of OXPHOS genes identified in our study is necessary to elucidate the mechanism mediating the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells.

中文翻译:

氧化石墨烯下调胶质母细胞瘤中氧化磷酸化复合物的基因。

最近,不同形式的纳米石墨烯被提出作为具有高抗癌潜力的材料。然而,石墨烯抑制癌症发展的机制仍不清楚。我们研究了含氧、还原和原始石墨烯对胶质母细胞瘤 U87 细胞系基因表达的影响。通过进行微阵列和 RT-qPCR 分析,我们发现氧化石墨烯(而不是还原氧化石墨烯和原始石墨烯)下调复合物 I、III、IV 和 V 的线粒体氧化磷酸化 (OXPHOS) 核基因的 mRNA 表达。为以下假设提供了第一个证据:GBM 生长受到抑制可能是 OXPHOS 蛋白表达下调和 ATP 水平降低的结果。我们认为,我们研究中发现的 OXPHOS 基因表达的变化可能介导氧化石墨烯在胶质母细胞瘤细胞中的抗增殖和抗迁移作用。然而,有必要对不同细胞系进一步研究我们研究中确定的 OXPHOS 基因的表达、调控和活性,以阐明介导氧化石墨烯在胶质母细胞瘤细胞中的抗增殖和抗迁移作用的机制。
更新日期:2019-01-03
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