Reference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC in paper disks of autochthonous strains, characterized by two, homogeneous and stable reference methods traceable at species level. RFC were produced using 14 regional species (7 yeasts and 7 filamentous fungi) from the fungal culture collection (DMic). Paper disks were impregnated with a culture suspension, dried and packed. Homogeneity, viability, identity and purity were verified. Short- and long-term stability at different temperatures and storage times were studied. Characterization of each strain allowed to confirm its identity and to ensure its traceability at international level. Produced batches were homogeneous and stable at −20 ± 5 °C for 30 months. This method of production was adequate to produce homogeneous and stable RFC with phenotypic and genotypic characteristics correctly defined and internationally traceable. Standardized procedures were developed for the production of certified RFC that could be transferred to other microorganisms. Providing RFC that represent regional strains allows laboratories to produce more reliable results with a favorable impact on medical diagnosis, the environment or the food industry.

参考真菌培养（RFC）对于实验室的内部质量控制至关重要。这些培养物的生产需要由公认和认可的实验室执行的标准化程序（IRAM 14950：2016和ISO 17034：2016标准）。这项工作的目的是在本地菌株的纸质圆盘中产生RFC，其特征是可在物种水平上溯源的两种均匀且稳定的参考方法。RFC是使用来自真菌培养物保藏中心（DMic）的14种区域性物种（7种酵母和7种丝状真菌）产生的。用培养悬浮液浸渍纸盘，干燥并包装。均一性，生存力，同一性和纯度得到验证。研究了在不同温度和储存时间下的短期和长期稳定性。对每种菌株的鉴定可以确认其身份并确保其在国际水平上的可追溯性。所生产的批次是均匀的，并且在-20±5°C下稳定30个月。这种生产方法足以产生具有正确定义和国际可追溯的表型和基因型特征的均质和稳定的RFC。开发了用于产生可转移到其他微生物的认证RFC的标准化程序。提供代表区域应变的RFC，可使实验室产生更可靠的结果，并对医学诊断，环境或食品工业产生有利影响。这种生产方法足以产生具有正确定义和国际可追溯的表型和基因型特征的均质和稳定的RFC。开发了用于产生可转移到其他微生物的认证RFC的标准化程序。提供代表区域应变的RFC，可使实验室产生更可靠的结果，并对医学诊断，环境或食品工业产生有利影响。这种生产方法足以产生具有正确定义和国际可追溯的表型和基因型特征的均质和稳定的RFC。开发了用于产生可转移到其他微生物的认证RFC的标准化程序。提供代表区域应变的RFC，可使实验室产生更可靠的结果，并对医学诊断，环境或食品工业产生有利影响。