Getting into a weakened organism, non-tuberculosis mycobacteria (NTMB) contact not only with the cells of the microorganism but also with the microflora of the human body; however, these interactions are poorly understood. The purpose of this work was to study the effect of NTMB supernatants on the properties of conditionally pathogenic microorganisms in their interaction with red blood cells. We used strains of non-tuberculous mycobacteria Mycobacterium iranicum and M. rutilum, as well as strains of Staphylococcus epidermidis and Escherichia coli. Using the fluorescence in situ hybridisation (FISH) method, the processes of adhesion to the surface of erythrocytes and the intra-erythrocyte penetration of cells of S. epidermidis and E. coli under the influence of NTMB supernatants were studied. To study changes in the haemoglobin molecule under the action of the supernatants of NTMB, spectral analysis was performed. Statistical processing was performed using STATISTIKA 6.0. The results showed that the supernatants of M. iranicum and M. rutilum increased the adhesion of conditionally pathogenic bacteria with a low level of AntiHbA to the surface of erythrocytes by 3-4 times. It also increased the intra-erythrocyte penetration of cells of S. epidermidis and E. coli relative to the control values. As a result of studying the haemoglobin spectrum of erythrocytes under the influence of M. iranicum, a decrease in the optical density values of oxyhaemoglobin by a factor of 2 relative to the values in the control sample was noted. Thus, the supernatants of NTMB have a multidirectional effect on the interaction of opportunistic microorganisms with erythrocytes, increasing the adhesive activity and the penetration of cells into the erythrocytes, as well as reducing the optical density of oxyhaemoglobin.

中文翻译：

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非结核分枝杆菌对机会性微生物与红细胞相互作用的影响。

非结核分枝杆菌（NTMB）进入弱化的生物体不仅与微生物细胞接触，而且与人体微生物区系接触；但是，这些相互作用的了解甚少。这项工作的目的是研究NTMB上清液对条件病原微生物与红细胞相互作用的特性的影响。我们使用了非结核分枝杆菌拉美支原体和r。Mutilus，以及表皮葡萄球菌和大肠杆菌的菌株。利用荧光原位杂交（FISH）方法，研究了NTMB上清液对表皮葡萄球菌和大肠杆菌细胞粘附在红细胞表面的过程以及红细胞在细胞内的渗透过程。为了研究在NTMB上清液的作用下血红蛋白分子的变化，进行了光谱分析。使用STATISTIKA 6.0进行统计处理。结果显示，M。iranicum和M. rutilum的上清液将具有低AntiHbA水平的条件致病菌与红细胞表面的粘附力提高了3-4倍。相对于对照值，它还增加了表皮葡萄球菌和大肠杆菌细胞的红细胞内渗透。研究了在M. iranicum的影响下红细胞的血红蛋白光谱，结果发现氧合血红蛋白的光密度值相对于对照样品的值降低了两倍。从而，