The purpose of this study was to assess the antioxidant and anti-inflammatory potential of liposomal formulations enriched with Hypericum hookerianum (Hyp) aqueous extracts. Cotyledon segments derived from protocorms of H. hookerianum were cultured on Murashige and Skoog (MS) media supplemented with Kinetin (KN, 1 mgl⁻¹) and Naphthalene acetic acid (NAA, 0.1 mgl⁻¹) to induce hypericin-rich red shoots (HypR, 0.87 mg/G DW). Highly stable liposomes (−29.4 mV) were successfully developed which encapsulated 63 ± 0.8% Hyp extracts, respectively. MTT assay subsequently confirmed the biocompatibility of liposome compositions using fibroblast cell lines. This work also evaluated acute toxicity of L-HypR and L-HypG formulations using Danio rerio (Zebrafish) embryos for 96 hpf. The expression of antioxidant and anti-inflammatory genes were found to be upregulated for L-HypR than L-HypG (green shoots without hypericin) formulations. These properties of L-HypR may be extremely useful for incorporating lipophilic substances into the food or pharmaceutical industry.

这项研究的目的是评估富含贯叶连翘（Hyp）水提取物的脂质体制剂的抗氧化和抗炎潜力。从球茎衍生子叶段H.虎头上Murashige和补充有激动素（KN，1个MGL斯库格（MS）培养基中培养^-1）和萘乙酸（NAA，0.1 MGL ^-1）来诱导金丝桃素浓红芽（ HypR，0.87 mg / G DW）。成功开发了高度稳定的脂质体（-29.4 mV），分别包裹了63±0.8％的Hyp提取物。随后，MTT测定法使用成纤维细胞系证实了脂质体组合物的生物相容性。这项工作还使用以下方法评估了L-HypR和L-HypG制剂的急性毒性Danio rerio（Zebrafish）胚胎为96 hpf。发现抗氧化剂和抗炎基因的表达相对于L-HypG（不含金丝桃素的绿芽）制剂而言，L-HypR的表达上调。L-HypR的这些特性对于将亲脂性物质掺入食品或制药行业中可能非常有用。