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Upregulation of glucose and amino acid transporters in micropapillary carcinoma.
Histology and Histopathology ( IF 2 ) Pub Date : 2019-03-11 , DOI: 10.14670/hh-18-099
Kanae Nosaka 1, 2 , Karen Makishima 2 , Tomohiko Sakabe 1 , Yohei Yurugi 3 , Makoto Wakahara 3 , Yasuaki Kubouchi 3 , Yasushi Horie 2 , Yoshihisa Umekita 1, 2
Affiliation  

Micropapillary carcinoma (MPC), a relatively rare histologic carcinoma observed in various organs, is associated with vascular invasion, nodal metastasis, and poor prognosis. MPC is different from papillary carcinoma as it has no fibrovascular core and is thus considered essentially hypovascular. MPCs are known to upregulate glucose transporter 1 (GLUT1) via the activation of a transcription factor, hypoxia-inducible factor (HIF)-1. Here we evaluated the expression of nutrient transporters in MPCs to gain a better understanding of the system used by MPCs to compensate for their intrinsic poor vascularity. We immunohistochemically evaluated 29 MPCs including breast (n=14), lung (n=8), gastrointestinal tract (n=5), and urinary tract cancers (n=2), and compared them with non-micropapillary control cancers (n=32) regarding the expression of amino acid (ASCT1, ASCT2, LAT1, and SNAT1) and glucose (GLUT1, GLUT2) transporters. Each section was scored by the staining intensity (0-3) multiplied by the occupying area (0-10), with a possible range 0-30. The average scores of the MPC and control groups were compared by Student's or Welch's t-test according to the homoscedasticity. The MPC group showed significantly higher scores for ASCT1 (p=0.007), ASCT2 (p=0.001), GLUT1 (p<0.001), and GLUT2 (p<0.001), whereas no significant scores were noted for LAT1 and SNAT1. In conclusion, MPC could be associated with the upregulation of several nutrient transporters, which may contribute to the malignant potential by supporting the survival of cancer cells.

中文翻译:

微乳头状癌中葡萄糖和氨基酸转运蛋白的上调。

微乳头状癌(MPC)是一种在各种器官中观察到的相对罕见的组织学癌,与血管浸润,淋巴结转移和预后不良有关。MPC与乳头状癌不同,因为它没有纤维血管核心,因此被认为本质上是血管不足的。已知MPC通过激活转录因子低氧诱导因子(HIF)-1来上调葡萄糖转运蛋白1(GLUT1)。在这里,我们评估了MPC中营养转运蛋白的表达,以更好地了解MPC用来补偿其固有的不良血管性的系统。我们通过免疫组织化学方法评估了29个MPC,包括乳腺癌(n = 14),肺癌(n = 8),胃肠道(n = 5)和泌尿道癌(n = 2),并将它们与非微乳头状癌(n = 32)的氨基酸(ASCT1,ASCT2,LAT1和SNAT1)和葡萄糖(GLUT1,GLUT2)转运蛋白的表达进行比较。用染色强度(0-3)乘以占用面积(0-10)对每个部分评分,可能范围为0-30。MPC和对照组的平均分数根据均方差通过Student或Welch's t检验进行比较。MPC组显示出ASCT1(p = 0.007),ASCT2(p = 0.001),GLUT1(p <0.001)和GLUT2(p <0.001)的得分明显较高,而LAT1和SNAT1则没有显着得分。总之,MPC可能与几种营养转运蛋白的上调有关,这可能通过支持癌细胞的存活而增加了恶性潜能。和SNAT1)和葡萄糖(GLUT1,GLUT2)转运蛋白。用染色强度(0-3)乘以占用面积(0-10)对每个部分评分,可能范围为0-30。MPC和对照组的平均分数根据均方差通过Student或Welch's t检验进行比较。MPC组显示出ASCT1(p = 0.007),ASCT2(p = 0.001),GLUT1(p <0.001)和GLUT2(p <0.001)的得分明显较高,而LAT1和SNAT1则没有显着得分。总之,MPC可能与几种营养转运蛋白的上调有关,这可能通过支持癌细胞的存活而增加了恶性潜能。和SNAT1)和葡萄糖(GLUT1,GLUT2)转运蛋白。用染色强度(0-3)乘以占用面积(0-10)对每个部分评分,可能范围为0-30。MPC和对照组的平均分数根据均方差通过Student或Welch's t检验进行比较。MPC组显示出ASCT1(p = 0.007),ASCT2(p = 0.001),GLUT1(p <0.001)和GLUT2(p <0.001)的得分明显较高,而LAT1和SNAT1则没有显着得分。总之,MPC可能与几种营养转运蛋白的上调有关,这可能通过支持癌细胞的存活而增加了恶性潜能。MPC和对照组的平均分数根据均方差通过Student或Welch's t检验进行比较。MPC组显示出ASCT1(p = 0.007),ASCT2(p = 0.001),GLUT1(p <0.001)和GLUT2(p <0.001)的得分明显较高,而LAT1和SNAT1则没有显着得分。总之,MPC可能与几种营养转运蛋白的上调有关,这可能通过支持癌细胞的存活而增加了恶性潜能。MPC和对照组的平均分数根据均方差通过Student或Welch's t检验进行比较。MPC组显示出ASCT1(p = 0.007),ASCT2(p = 0.001),GLUT1(p <0.001)和GLUT2(p <0.001)的得分明显较高,而LAT1和SNAT1则没有显着得分。总之,MPC可能与几种营养转运蛋白的上调有关,这可能通过支持癌细胞的存活而增加了恶性潜能。
更新日期:2020-08-21
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