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The first report of polymorphisms and genetic characteristics of the prion protein gene (PRNP) in horses.
Prion ( IF 2.3 ) Pub Date : 2018-09-12 , DOI: 10.1080/19336896.2018.1513316
Yong-Chan Kim 1, 2 , Byung-Hoon Jeong 1, 2
Affiliation  

Prion diseases have a wide host range, but prion-infected cases have never been reported in horses. Genetic polymorphisms that can directly impact the structural stability of horse prion protein have not been investigated thus far. In addition, we noticed that previous studies focusing on horse-specific amino acids and secondary structure predictions of prion protein were performed for limited parts of the protein. In this study, we found genetic polymorphisms in the horse prion protein gene (PRNP) in 201 Thoroughbred horses. The identified polymorphism was assessed to determine whether this polymorphism impedes stability of protein using PolyPhen-2, PROVEAN and PANTHER. In addition, we evaluated horse-specific amino acids in horse and mouse prion proteins using same methods. We found only one single nucleotide polymorphism (SNP) in the horse prion protein, and three annotation tools predicted that the SNP is benign. In addition, horse-specific amino acids showed different effects on horse and mouse prion proteins, respectively.

Abbreviations: PRNP: prion protein gene; SNP: single nucleotide polymorphism; CJD: Creutzfeldt-Jakob disease; CWD: chronic wasting disease; TME: transmissible mink encephalopathy; FSE: feline spongiform encephalopathy; MD: molecular dynamics; ER: endoplasmic reticulum; GPI: glycosylphosphatidylinositol; NMR: nuclear magnetic resonance; ORF: open reading frame; GWAS: genome-wide association study; NAPA: non-adaptive prion amplification; HMM: hidden Markov model; NCBI: National Center for Biotechnology Information



中文翻译:

马的the病毒蛋白基因(PRNP)的多态性和遗传特征的首次报道。

on病毒疾病的宿主范围很广,但是从未有马感染reported病毒感染的病例。迄今为止,尚未直接影响马病毒蛋白结构稳定性的遗传多态性。此外,我们注意到先前针对马的特定氨基酸和病毒蛋白质二级结构预测的研究仅针对蛋白质的有限部分进行。在这项研究中,我们发现了pr病毒蛋白基因(PRNP)在201匹纯种马中。使用PolyPhen-2,PROVEAN和PANTHER对鉴定出的多态性进行评估,以确定该多态性是否会阻碍蛋白质的稳定性。此外,我们使用相同的方法评估了马和小鼠病毒蛋白中的马特异性氨基酸。我们在horse病毒蛋白中仅发现一个单核苷酸多态性(SNP),并且三个注释工具预测该SNP是良性的。另外,马特异性氨基酸分别对马和小鼠病毒蛋白显示不同的作用。

缩写PRNP:病毒蛋白基因;SNP:单核苷酸多态性;CJD:克雅氏病;CWD:慢性消耗性疾病;TME:可传播的水貂脑病;FSE:猫海绵状脑病;MD:分子动力学;ER:内质网;GPI:糖基磷脂酰肌醇;NMR:核磁共振;ORF:开放阅读框;GWAS:全基因组关联研究;NAPA:非自适应病毒扩增;HMM:隐马尔可夫模型;NCBI:国家生物技术信息中心

更新日期:2018-09-12
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