Background and purpose: The present study aimed to explore the feasibility and efficacy of the targeted non-invasive implantation of mesenchymal stromal cells (MSCs) by low-intensity ultrasound-targeted microbubble destruction (LI-UTMD) assisted blood-brain barrier (BBB) opening and its improvement on neurobehavioural outcomes in brain ischaemic rats.Methods: A transcranial irradiation of low-intensity ultrasound by diagnostic devices was performed, and lipid microbubbles (MBs) and MSCs were simultaneously infused. Then, the MSC transmigration from brain vessels to parenchyma was demonstrated, and MSCs were statistically analysed on days 1, 4, 7 and 14. Behavioural function was statistically analysed.Results: The extra-vascular leakage of lanthanum and EB was observed at the brain ischaemic area receiving ultrasound. MSCs were observed at the ultrasound irradiated brain hemisphere, and the number of MSCs in LI-UTMD assisted MSCs group was significantly higher than that in the MSCs group (p < .01). The attachment, traversing and trans-migration of MSCs across the BBB were recorded. Neuro-behavioural function was improved with this approach.Conclusions: The transcranial irradiation of low intensity ultrasound targeted MBs destruction on brain ischaemic rats might be a safe and efficient BBB opening approach to prompt the successful delivery of MSCs into the targeted area of brain ischaemia, and ameliorate neurological function.

中文翻译：

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低强度超声靶向微泡破坏有助于 MSCs 递送并改善脑缺血大鼠的神经功能。

背景与目的：本研究旨在探讨低强度超声靶向微泡破坏（LI-UTMD）辅助血脑屏障（BBB）无创靶向植入间充质基质细胞（MSCs）的可行性和有效性。开放及其对脑缺血大鼠神经行为结果的改善。方法：通过诊断装置进行低强度超声经颅照射，同时注入脂质微泡（MBs）和MSCs。然后，证明了MSC从脑血管向实质的迁移，并在第1、4、7和14天对MSC进行了统计分析。对行为功能进行了统计分析。结果：在脑部观察到镧和EB的血管外渗漏接受超声的缺血区域。在超声照射的大脑半球观察到MSCs，LI-UTMD辅助MSCs组的MSCs数量显着高于MSCs组（p < .01）。记录了 MSC 在 BBB 上的附着、穿越和迁移。这种方法改善了神经行为功能。 结论：低强度超声靶向 MBs 对脑缺血大鼠的经颅照射可能是一种安全有效的 BBB 开放方法，以促进 MSCs 成功递送到脑缺血的目标区域，并改善神经功能。记录了 MSC 在 BBB 上的穿越和迁移。这种方法改善了神经行为功能。 结论：低强度超声靶向 MBs 对脑缺血大鼠的经颅照射可能是一种安全有效的 BBB 开放方法，以促进 MSCs 成功递送到脑缺血的目标区域，并改善神经功能。记录了 MSC 在 BBB 上的穿越和迁移。这种方法改善了神经行为功能。 结论：低强度超声靶向 MBs 对脑缺血大鼠的经颅照射可能是一种安全有效的 BBB 开放方法，以促进 MSCs 成功递送到脑缺血的目标区域，并改善神经功能。