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Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens.
Biomolecular NMR Assignments ( IF 0.9 ) Pub Date : 2019-05-22 , DOI: 10.1007/s12104-019-09899-6
Liliana R Teixeira 1 , Pilar C Portela 1 , Leonor Morgado 1 , David Pantoja-Uceda 2 , Marta Bruix 2 , Carlos A Salgueiro 1
Affiliation  

Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (1H, 13C and 15N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.

中文翻译:

细胞色素PccH的骨干分配,这是减少土壤细菌中的硫的微生物电合成的重要蛋白质。

微生物电合成是一种新兴的绿色技术,它探索特定种类的微生物驱动其代谢,以通过电极表面提供的电子产生氢或增值化学品的能力。当电子被提供给减少硫杆菌的生物膜时,细胞色素PccH表现出最大的转录增加。基因敲除实验表明,通过减少编码细胞色素PccH的基因,可以完全抑制电子向硫还原菌的转移。这确定了该蛋白质在硫还原菌微生物的电合成机制中的关键作用,目前尚不清楚。在这项工作中,我们介绍了主干(1H,13 C和15 N)和PccH在氧化状态下的血红素分配。获得的数据为鉴定和结构定位细胞色素PccH及其生理氧化还原伴侣之间的分子相互作用区域铺平了道路。
更新日期:2019-05-22
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