BACKGROUND On May 2017, a case of dengue serotype 1 was detected and confirmed through routine surveillance in a traveler returning from Kribi, a seaside town of Southern Cameroon. This study aimed at confirming the circulation of dengue virus (DENV) in Southern Cameroon. METHODS A cross sectional study was carried out in Londji near Kribi from June 21-25, 2017, by a joint team of Centre Pasteur of Cameroon and the Department of Diseases, Epidemics and Pandemics Control. Blood samples of consented participants were collected and tested for anti-D ENV IgM using an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA), and for the detection of Zika, dengue, or chikungunya viruses using Trioplex real-time reverse transcription-polymerase chain reaction (RT-PCR). DENV RNA-positive samples were serotyped using an end-point nested RT-PCR. RESULTS Ninety-one participants were enrolled, 50.55% (46/91) of them males. The mean age of the population was 30.71 years (±18.89). In total, 14.28% (13/91) of the participants had DENV infection (3 anti-DENV IgM positive and 10 DENV serotype 1 RT-PCR positive). CONCLUSION The detection of DENV serotype 1 in an autochthonous population during this survey is a confirmation that the seaside city of Kribi is a risk area for contracting dengue infection in Cameroon.

中文翻译：

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喀麦隆南部克里比农村地区爆发1型登革热病毒的调查：跨部门研究。

背景2017年5月，从喀麦隆南部海滨小镇克里比（Kribi）返回的旅行者通过例行监测发现并确诊了一例登革热血清型1例病例。这项研究旨在确认喀麦隆南部的登革热病毒（DENV）的传播。方法于2017年6月21日至25日在喀比隆附近的隆吉进行了一项横断面研究，该研究由喀麦隆巴斯德中心和疾病，流行病与大流行控制部联合开展。收集同意的参与者的血样，并使用IgM抗体捕获酶联免疫吸附测定（MAC-ELISA）测试抗D ENV IgM，并使用Trioplex实时逆转录检测Zika，登革热或基孔肯雅病毒-聚合酶链反应（RT-PCR）。使用终点嵌套式RT-PCR对DENV RNA阳性样品进行血清分型。结果共有91位参与者参加，其中50.55％（46/91）是男性。人口的平均年龄是30.71岁（±18.89）。总共有14.28％（13/91）的参与者患有DENV感染（3例抗DENV IgM阳性和10例DENV血清型1 RT-PCR阳性）。结论在本次调查中，在当地居民中检测到DENV血清型1证实了海滨城市Kribi是喀麦隆感染登革热的危险区域。