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Identification and characterization of splenic adherent cells forming densely-packed colonies.
Development, Growth & Differentiation ( IF 2.5 ) Pub Date : 2019-03-29 , DOI: 10.1111/dgd.12605
Masataka Hirasaki 1 , Yosuke Mizuno 2 , Yui Ida 3 , Takayuki Murakoshi 3 , Akihiko Okuda 1 , Norihiro Kotani 3
Affiliation  

It is thought that the spleen contains stem cells that differentiate into somatic cells other than immune cells. We investigated the presence of these hypothetical splenic cells with stem cell characteristics and identified adherent cells forming densely-packed colonies (Splenic Adherent Colony-forming Cell; SACC) in the spleen. Splenic Adherent Colony-forming Cell was positive for alkaline phosphatase staining and stage-specific embryonic antigen (SSEA)-1 antigen. However, the self-renewal properties of SACCs were limited because they stopped cell proliferation once colonies visible to the naked eye were formed. Gene expression analyses by semi-quantitative RT-PCR revealed the significant expression of c-Myc and Klf4, whereas faint or no expression was evident for Nanog, Oct3/4, and Sox2. Global expression analyses by DNA microarray and subsequent gene ontology analyses revealed that the expression levels of genes related to the immune system were significantly lower in SACCs than in control splenic cells. In contrast, genes unrelated to the immune system, such as those involved in cell adhesion and axon guidance, were relatively highly expressed in SACCs compared with control splenic cells. Taken together, we identified a novel cell type residing in the spleen that is different from the hypothetical splenic stem cell, but which bears some, but not all, characteristics that represent an undifferentiated state.

中文翻译:

鉴定和表征形成密集堆积菌落的脾黏附细胞。

据认为,脾脏含有分化成免疫细胞以外的体细胞的干细胞。我们调查了具有干细胞特征的这些假想脾细胞的存在,并鉴定了在脾脏中形成紧密堆积菌落的粘附细胞(脾黏附集落形成细胞; SACC)。脾脏粘附集落形成细胞对碱性磷酸酶染色和阶段特异性胚胎抗原(SSEA)-1抗原呈阳性。但是,SACC的自我更新特性受到限制,因为一旦形成肉眼可见的菌落,它们就会停止细胞增殖。通过半定量RT-PCR进行的基因表达分析揭示了c-Myc和Klf4的显着表达,而Nanog,Oct3 / 4和Sox2的表达则微弱或没有。DNA微阵列的整体表达分析和随后的基因本体分析表明,与免疫系统相关的基因的表达水平在SACC中显着低于对照脾细胞。相反,与免疫系统无关的基因,例如那些参与细胞粘附和轴突引导的基因,与对照组的脾细胞相比,在SACC中的表达相对较高。综上所述,我们确定了一种新的细胞类型,它位于脾脏中,不同于假定的脾干细胞,但具有一些代表全部未分化状态的特征,但并非全部。与对照组的脾细胞相比,诸如SACC中涉及细胞黏附和轴突引导的细胞等表达相对较高。综上所述,我们确定了一种新的细胞类型,它位于脾脏中,不同于假定的脾干细胞,但具有一些代表全部未分化状态的特征,但并非全部。与对照组的脾细胞相比,诸如SACC中涉及细胞黏附和轴突引导的细胞等表达相对较高。综上所述,我们确定了一种新的细胞类型,它位于脾脏中,不同于假定的脾干细胞,但具有一些代表全部未分化状态的特征,但并非全部。
更新日期:2019-11-01
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