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An in situ hybridization study of MMP-2, -9, -13, -14, TIMP-1, and -2 mRNA in fetal mouse mandibular condylar cartilage as compared with limb bud cartilage.
Gene Expression Patterns ( IF 1.2 ) Pub Date : 2019-02-27 , DOI: 10.1016/j.gep.2019.02.003
Masato Takahashi 1 , Kaoru Fujikawa 2 , Randilini Angammana 1 , Shunichi Shibata 1
Affiliation  

The main purpose of this in situ hybridization study was to investigate MMPs and TIMPs mRNA expression in developing mandibular condylar cartilage and limb bud cartilage. At E14.0, MMP-2, -14, TIMP-1 and -2 mRNAs were expressed in the periosteum of mandibular bone, and in the condylar anlage. At E15.0 MMP-2, -14, TIMP-1 and -2 mRNAs were expressed in the perichondrium of newly formed condylar cartilage and the periosteum of developing bone collar, whereas, expression of MMP-14 and TIMP-1 mRNAs were restricted to the inner layer of the periosteum/perichondrium. This expression patterns continued until E18.0. Further, from E13.0 to 14.0, in the developing tibial cartilage, MMP-2, -14, and TIMP-2 mRNAs were expressed in the periosteum/perichondrium, but weak MMP-14 and no TIMP-1 mRNA expression was recognized in the perichondrium. These results confirmed that the perichondrium of condylar cartilage has characteristics of periosteum, and suggested that MMPs and/or TIMPs are more actively involved in the development of condylar (secondary) cartilage than tibial (primary) cartilage. MMP-9-positive cells were observed in the bone collar of both types of cartilage, and they were consistent with osteoclasts/chondroclasts. MMP-13 mRNA expression was restricted to the chondrocytes of the lower hypertrophic cell zone in tibial cartilage at E14.0, indicating MMP-13 can be used as a marker for lower hypertrophic cell zone. It was also expressed in chondrocytes of newly formed condylar cartilage at E15.0, and continuously expressed in the lower hypertrophic cell zone until E18.0. These results confirmed that progenitor cells of condylar cartilage are rapidly differentiated into hypertrophic chondrocytes, which is a unique structural feature of secondary cartilage different from that of primary cartilage.



中文翻译:

与肢芽软骨相比,胎儿小鼠下颌con突软骨中MMP-2,-9,-13,-14,TIMP-1和-2 mRNA的原位杂交研究。

这项原位杂交研究的主要目的是研究MMPsTIMPs mRNA在下颌con突软骨和四肢芽软骨发育中的表达。在E14.0,MMP-2-14TIMP-1-2 mRNA在下颌骨骨膜和,突中表达。在E15.0时,MMP-2-14TIMP-1-2 mRNA在新形成的con突软骨的软骨膜和发育中的骨环的骨膜中表达,而MMP-14TIMP-1的表达mRNA被限制在骨膜/软骨膜的内层。这种表达模式一直持续到E18.0。此外,从E13.0到14.0,在发育中的胫骨软骨中,骨膜/软骨膜中表达了MMP-2-14TIMP-2 mRNA,但在骨骼肌中膜MMP-14弱且未识别到TIMP-1 mRNA表达。软骨膜。这些结果证实that突软骨的软骨膜具有骨膜的特征,并且表明MMP和/或TIMP比t胫骨(初级)软骨更活跃地参与con突(次级)软骨的发育。MMP-9在两种类型的软骨的骨环中都观察到阳性细胞,它们与破骨细胞/软骨细胞一致。在E14.0时,MMP-13 mRNA的表达仅限于胫骨软骨下部肥大细胞区的软骨细胞,表明MMP-13可用作下部肥大细胞区的标志物。它也在新形成的newly突软骨的软骨细胞中在E15.0表达,并在下部肥大细胞区连续表达直到E18.0。这些结果证实con突软骨的祖细胞迅速分化为肥大的软骨细胞,这是继发软骨与原发软骨不同的独特结构特征。

更新日期:2019-02-27
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