Delta GST is an insect‐specific class and a prominent class of the glutathione S‐transferases family that is involved in xenobiotic detoxification and antioxidant defense. The full‐length complementary DNA of delta‐class GST from Penaeus monodon (PmDeltaGST; 839 bp long with a 657 bp coding region) was cloned. The encoded polypeptide of 218 amino acids had a predicted molecular mass of 24.30 kDa. Sequence homology and phylogenetic analysis showed that PmDeltaGST was significant similarity to GST genes in crustaceans and insects. Tissue expression profile analysis by quantitative real‐time reverse‐transcription polymerase chain showed that PmDeltaGST was constitutively expressed in all the examined tissues, with the highest expression in hepatopancreas and intestine and the weakest expression in ovary. PmDeltaGST messenger RNA expression and protein levels in hepatopancreas was significantly increased at 14 days postexposure of aflatoxin B1 (AFB1), keeping on the high level at 28 days, but decreased at 56 days. The results suggested that PmDeltaGST was involved in the response to AFB1 exposure.

中文翻译：

---

暴露于黄曲霉毒素B1（AFB1）的黑虎虾（斑节对虾）中Delta-GST的鉴定和表达谱。

Delta GST是谷胱甘肽S转移酶家族中一个昆虫特有的一类，是杰出的一类，它参与异种生物的解毒和抗氧化防御。克隆了斑节对虾（PmDeltaGST；长839 bp，编码区域657 bp）的三角洲GST的全长互补DNA 。编码的218个氨基酸的多肽的预测分子量为24.30 kDa。序列同源性和系统发育分析表明，PmDeltaGST与甲壳类和昆虫中的GST基因具有显着相似性。通过定量实时逆转录聚合酶链分析组织表达谱表明，PmDeltaGST在所有检查的组织中组成性表达，在肝胰腺和肠道中表达最高，在卵巢中表达最弱。黄曲霉毒素B1（AFB1）暴露后14天，肝胰腺中PmDeltaGST信使RNA表达和蛋白质水平显着增加，在28天时保持较高水平，但在56天时下降。结果表明PmDeltaGST参与了对AFB1暴露的反应。