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Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia.
Immunologic Research ( IF 4.4 ) Pub Date : 2019-06-01 , DOI: 10.1007/s12026-019-09078-8
Priscila R Nunes 1 , Mariana Romão-Veiga 1 , José C Peraçoli 2 , Roberto A Araujo Costa 2 , Leandro G de Oliveira 2 , Vera Therezinha M Borges 2 , Maria Terezinha Peraçoli 1
Affiliation  

Preeclampsia (PE) is a pregnancy-specific syndrome characterized by a systemic inflammatory response that polarizes peripheral blood monocytes to the M1 phenotype. The classically activated M1 monocytes comprise immune effector cells with an acute inflammatory phenotype. CD163 is a scavenger receptor expressed by monocytes/macrophages that may be shed from their cell membrane after proteolytic cleavage, producing the soluble CD163 molecule (sCD163). This study evaluated CD163 expression by monocytes and sCD163 as well as pro- and anti-inflammatory cytokine concentration in the plasma of pregnant women with PE. Fifty-six women with PE and 28 normotensive pregnant women were included. Plasma levels of sCD163, interleukin-1 beta (IL-1β), IL-6, IL-10, transforming growth factor beta (TGF-β1), and tumor necrosis factor-alpha (TNF-α) were determined by ELISA, and CD163 expression by monocytes was assessed by flow cytometry. The expression of CD163 by monocytes was significantly lower in severe and mild PE than in normotensive pregnant. Plasma concentrations of IL-1β, TGF-β1, and TNF-α were higher in severe PE than in mild PE and normotensive pregnant women. Both groups of preeclamptic women showed decreased plasma levels of sCD163 and IL-10. Negative correlations between sCD163 and IL-1β (r = - 0.45; P = 0.014) and between sCD163 and TNF-α concentrations (r = - 0.54; P = 0.001) were observed in the severe PE group. The association between the pro-inflammatory cytokine profile and lower concentrations of sCD163 and IL-10 in plasma from women with severe PE suggests an impairment in the modulation of the systemic inflammatory response in this group of pregnant women with preeclampsia.

中文翻译:

在子痫前期孕妇中,单核细胞中CD163的下调及其在血浆中的可溶形式与促炎特性有关。

子痫前期(PE)是一种妊娠特异性综合征,其特征是全身性炎症反应,使外周血单核细胞极化至M1表型。经典活化的M1单核细胞包括具有急性炎症表型的免疫效应细胞。CD163是由单核细胞/巨噬细胞表达的清除剂受体,在蛋白水解切割后可能从其细胞膜上脱落,从而产生可溶性CD163分子(sCD163)。这项研究评估了PE孕妇血浆中单核细胞和sCD163的CD163表达以及促炎和抗炎细胞因子的浓度。包括56名PE妇女和28名血压正常的孕妇。血浆sCD163,白介素-1β(IL-1β),IL-6,IL-10,转化生长因子β(TGF-β1),ELISA法检测肿瘤坏死因子-α(TNF-α),流式细胞仪评估单核细胞CD163表达。重度和轻度PE中单核细胞CD163的表达显着低于血压正常的孕妇。重度PE患者的血浆IL-1β,TGF-β1和TNF-α浓度高于轻度PE和血压正常的孕妇。两组先兆子痫妇女的血浆sCD163和IL-10水平均降低。在严重PE组中,sCD163和IL-1β之间(r =-0.45; P = 0.014)以及sCD163和TNF-α浓度之间(r =-0.54; P = 0.001)呈负相关。
更新日期:2019-11-01
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