ABSTRACT Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca2+-activated Cl− channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.

中文翻译：

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Anoctamin-1影响未分化甲状腺癌细胞的迁移和侵袭

摘要甲状腺未分化癌（ATC）是一种罕见的恶性肿瘤，预后极差。其强烈攻击性的确切原因尚不清楚。在这里，我们发现晚期 ATC 组织中 anoctamin-1（ANO1；Ca2+ 激活的 Cl− 通道）的表达升高。使用不同的 ATC 细胞系，通过定量逆转录聚合酶链反应和蛋白质印迹发现 ANO1 的表达程度与 ATC 细胞侵袭的程度有关。通过选择性抑制剂 (T16Ainh-A01) 或 siRNA 抑制 ANO1 活性显着减弱了 ATC 细胞的迁移和侵袭。总之，ANO1 似乎增加了 ATC 细胞侵入和迁移的能力。我们的研究结果还表明 ANO1 在 ATC 患者中的表达可能有助于预测 ATC 的预后。