BACKGROUND Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos. RESULTS Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression. CONCLUSION Our work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.

中文翻译：

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单细胞RNA测序揭示了小鼠胚胎的光诱导损伤。

背景技术在生殖中心的胚胎的体外操作中，曝光是常见的应激因素。许多研究表明高强度光照对不同动物胚胎的有害影响。但是，没有转录组学研究探索光诱导的损伤和植入前胚胎的反应。结果在这里，我们采用不同的时程和光照强度来处理2细胞阶段的小鼠胚胎，并评估它们对囊胚形成的影响。同时，在高强度光照后分析了从2细胞到胚泡期的单细胞转录组。这些数据表明，每个胚胎阶段的细胞都可以分为不同的光照条件。差异表达基因和GO术语的进一步分析揭示了高强度照明后的光诱导损伤以及潜在的修复反应。母体向合子的过渡也受到无法去除母体RNA和使合子基因组表达失活的影响。结论我们的工作揭示了对高强度照明的综合反应，涉及形态变化，长期伤害作用和细胞内损伤修复机制。