The aim of this study is to detect the dynamic expression of interleukin-23 (IL-23) in ApoE-/- mice at different ages and to further examine the effects of anti-IL-23 therapy on atherosclerosis development. The levels of IL-23 in the sera, aortas, and lymph nodes of ApoE-/- mice were significantly increased compared with those of age-matched controls at 8, 12, 16, 20, and 24 weeks of age. Then, 12-week-old ApoE-/- mice were intraperitoneally injected with anti-IL-23p19 neutralizing antibodies, isotype controls, and phosphate-buffered saline for 8 weeks. The proinflammatory and anti-inflammatory mediators in atherosclerotic aortas, plaque areas, plaque necrotic cores, and the contents of major inflammatory cells in plaques were subsequently determined. The results showed that anti-IL-23p19 treatment significantly decreased the expression of IL-17A, IL-6, and TNF-α in the aortas of ApoE-/- mice, but had no obvious effect on the plaque area, plaque necrotic core, or content of major inflammatory cells in atherosclerotic plaques. Although anti-IL-23p19 therapy reduces the expression of several proinflammatory cytokines, it does not significantly suppress the progression of atherosclerosis in ApoE-/- mice.

中文翻译：

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抗IL-23p19治疗对ApoE-/-小鼠动脉粥样硬化发展的影响。

这项研究的目的是检测不同年龄的ApoE-/-小鼠中白细胞介素23（IL-23）的动态表达，并进一步检查抗IL-23治疗对动脉粥样硬化发展的影响。与年龄匹配的对照组相比，在8、12、16、20和24周龄时，ApoE-/-小鼠的血清，主动脉和淋巴结中的IL-23水平显着增加。然后，腹膜内注射12周龄的ApoE-/-小鼠抗IL-23p19中和抗体，同型对照和磷酸盐缓冲液，持续8周。随后确定了动脉粥样硬化主动脉，斑块区域，斑块坏死核心中的促炎和抗炎介质，以及斑块中主要炎症细胞的含量。结果表明，抗IL-23p19处理可显着降低IL-17A的表达，IL-6和TNF-α在ApoE-/-小鼠的主动脉中，但对斑块面积，斑块坏死核心或动脉粥样硬化斑块中主要炎症细胞的含量没有明显影响。尽管抗IL-23p19疗法可减少几种促炎细胞因子的表达，但它并未显着抑制ApoE-/-小鼠的动脉粥样硬化进展。