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Molecular identification of biological contaminants in different drinking water resources of the Jazan region, Saudi Arabia.
Journal of Water & Health ( IF 2.3 ) Pub Date : 2019-07-18 , DOI: 10.2166/wh.2019.019
Emad Abada 1 , Zarraq Al-Fifi 2 , Abdul Jabbar Al-Rajab 3 , Mosbah Mahdhi 4 , Mukul Sharma 4
Affiliation  

Drinking water quality plays a remarkable role in human infections and diseases. This study used polymerase chain reaction (PCR) techniques to detect bacterial pathogens. In addition, a physicochemical analysis was performed on drinking water samples from several sources. A total of 123 drinking water samples were collected from different areas in the Jazan region in Saudi Arabia: ground water (40 samples), bottled water (15 samples), tap water (52 samples), and water purification shops (16 samples). To isolate the bacterial pathogens, the water samples were spread on Nutrient and MacConkey agar media, and the grown pathogens were then identified by the 16S ribosomal RNA technique. In 87 (70.7%) of the 123 drinking water samples, there was no pathogen growth on the two-culture medium. However, 36 (29.3%) of the samples were found to be contaminated with bacteria. The physicochemical analysis indicated that the water samples were within the Saudi drinking water standards. The bacteria were resistant to Cefotaxime, Cefotaxime/Clavulanic acid, Erythromycin, Penicillin G, Rifampin and Sulfamethoxazole-Trimethoprim, respectively. The findings suggest that in Jazan, bottled water is a safer source of potable water than tap water. The contamination in the water may be occurring at the reservoirs rather than the water sources.

中文翻译:

沙特阿拉伯贾赞地区不同饮用水资源中生物污染物的分子鉴定。

饮用水水质在人类感染和疾病中起着重要作用。这项研究使用聚合酶链反应(PCR)技术来检测细菌病原体。此外,还对几种来源的饮用水样品进行了理化分析。从沙特阿拉伯Jazan地区不同地区收集了总共123个饮用水样品:地下水(40个样品),瓶装水(15个样品),自来水(52个样品)和净水店(16个样品)。为了分离细菌病原体,将水样分散在营养和MacConkey琼脂培养基上,然后通过16S核糖体RNA技术鉴定生长的病原体。在123个饮用水样本中的87个(70.7%)中,两种培养基中均没有病原体生长。但是,36(29。发现3%的样品被细菌污染。理化分析表明水样在沙特饮用水标准内。该细菌分别对头孢噻肟,头孢噻肟/克拉维酸,红霉素,青霉素G,利福平和磺胺甲恶唑-甲氧苄啶具有抗性。研究结果表明,在贾赞邦,瓶装水比自来水更安全。水中的污染可能发生在水库而不是水源处。瓶装水比自来水更安全。水中的污染可能发生在水库而不是水源处。瓶装水比自来水更安全。水中的污染可能发生在水库而不是水源处。
更新日期:2019-11-01
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