In vivo oxygen levels in tissues range from 1% to 15%, while mechanistic cell culture studies employ an atmospheric oxygen level of 21% to grow cells. These oxygen concentrations are therefore not representative for conditions where the cell response is dependent on oxygen partial pressure. In pathological situation, such as (colon) cancer or chronic inflammation, tissue oxygenation is severely affected, and even under physiological conditions a steep oxygen gradient is present in the large intestine, where epithelial cells co‐exist with microbial species, resulting in almost anoxia at the midpoint of the lumen. In these situations, a better characterisation of the essential cellular behaviour under hypoxia or anoxia is required.

中文翻译：

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五种肠道细胞系对体外缺氧条件的反应

组织中的体内氧含量范围从 1% 到 15%，而机械细胞培养研究采用 21% 的大气氧含量来培养细胞。因此，这些氧浓度不代表细胞响应取决于氧分压的条件。在病理情况下，例如（结肠）癌或慢性炎症，组织氧合受到严重影响，甚至在生理条件下，大肠中也存在陡峭的氧梯度，其中上皮细胞与微生物物种共存，导致几乎缺氧在管腔的中点。在这些情况下，需要更好地表征缺氧或缺氧下的基本细胞行为。