Scopelophila ligulata is an Fe-hyperaccumulator moss growing in acidic environments, but the mechanism of Fe accumulation remains unknown. To understand the mechanism, we determined Fe species in S. ligulata samples. The moss samples were collected from four sites in Japan. The concentrations of Fe, P, S, Cl, and K in them were measured by induced coupled plasma mass spectrometry. Fe species in some of them were determined by Mössbauer spectroscopy and were confirmed by X-ray diffraction analysis. Fe species in S. ligulata samples were determined to be jarosite, ferritin, high-spin Fe(II) species, and akaganeite. To our knowledge, this is the first report on the biomineralization of jarosite in mosses. This result, combined with the fact that bacteria, a fungus, and a grass mineralize jarosite, suggests that its biomineralization is a common characteristic in a wide variety of living organisms. These findings indicate that the biomineralization of jarosite occurs not only in the region-specific species but in species adapted to a low-pH and metal-contaminated environment in different regions, provide a better understanding of the mechanism of Fe accumulation in the Fe-hyperaccumulator moss S. ligulata, and offer new insights into the biomineralization of jarosite.

中文翻译：

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通过Mössbauer，X射线衍射和元素分析法测定超级富集生苔藓Scopelophila ligulata中铁的种类，包括生物矿化的黄铁矿。

Scopelophila ligulata是一种在酸性环境中生长的铁超积累型苔藓，但铁的积累机理尚不清楚。为了了解其机理，我们确定了S. ligulata样品中的Fe种类。苔藓样本是从日本的四个地点收集的。通过感应耦合等离子体质谱法测量其中的Fe，P，S，Cl和K的浓度。其中一些中的Fe种类通过Mössbauer光谱确定，并通过X射线衍射分析确定。ligulata样品中的Fe种类被确定为黄铁矿，铁蛋白，高旋转Fe（II）种类和赤铁矿。据我们所知，这是关于苔藓中黄钾铁矾生物矿化的首次报道。这个结果与细菌，真菌和草使黄铁矿矿化的事实结合在一起，提示其生物矿化是多种活生物体的共同特征。这些发现表明，黄钾铁矾的生物矿化不仅发生在特定区域的物种中，而且还发生在适应低pH值和金属污染环境的物种中，从而更好地了解了Fe超级蓄积物中Fe积累的机理。 moss S. ligulata，并为黄铁矿的生物矿化提供新见解。