To consolidate the genetic, physical, and cytogenetic maps of scallop (Patinopecten yessoensis), we constructed a molecular cytogenetic map by localizing 84 fosmid clones that contain different SNP markers from 19 linkage groups (LGs) using fluorescence in situ hybridization (FISH). Among these 84 SNP-anchored clones, 56 clones produced specific and stable signals on one pair of chromosomes. Dual-color FISH assigned 19 LGs to their corresponding chromosomes with 38 SNP-anchored clones as probes. Among these 19 LGs, 17 LGs were assigned to their corresponding one pair of chromosomes, while two clones containing SNPs from LG10 and LG19 were located on two different pairs of chromosomes separately. The orientation of 7 LGs was corrected according to the chromosome location of SNPs within the same LG. In addition, a probe panel of SNP-anchored clones was developed to identify each chromosome of P. yessoensis. The molecular cytogenetic map will facilitate molecular breeding in scallop and enable comparative studies on chromosome evolution of bivalve mollusk.

中文翻译：

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扇贝（Patinopecten yessoensis）的分子细胞遗传学图。

巩固扇贝的遗传，物理和细胞遗传图谱（Patinopecten yessoensis），我们使用荧光原位杂交（FISH）通过定位来自19个连锁组（LG）的84个含有不同SNP标记的fosmid克隆构建了分子细胞遗传学图谱。在这84个SNP锚定的克隆中，有56个克隆在一对染色体上产生了特定且稳定的信号。双色FISH用38个SNP锚定的克隆作为探针将19个LG分配给了它们对应的染色体。在这19个LG中，有17个LG被分配给它们对应的一对染色体，而含有LG10和LG19的SNP的两个克隆分别位于两个不同的染色体对上。根据同一个LG中SNP的染色体位置来校正7个LG的方向。此外，开发了一个SNP锚定克隆的探针组，以鉴定P. yessoensis的每个染色体。。分子细胞遗传学图谱将有助于扇贝的分子育种，并使对双壳软体动物染色体进化的比较研究成为可能。