Sulfated oligosaccharides are involved in important biological events that are often modulated by specific sequences and sulfation patterns, but their structural analysis remains challenging. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis of three different sulfated oligosaccharides (Fondaparinux, the octasulfated pentasaccharide, a disulfated heparin-derived tetrasaccharide 1, and an octasulfated maltoheptaose) 2 was performed using the 2-(4-hydroxyphenylazo)benzoic acid-tetramethylguanidinium (HABA-TMG2) matrix. High resolution mass spectrometry of the main ions observed was successful, and this was complemented by tandem mass spectrometry (MS/MS) analysis for structural assessment. Despite sulfate losses, fully sulfated molecular ions were observed and these allowed the determination of oligosaccharide structures: UA-GlcNAc-UA(2S)-AnhMan(6S) for compound 1 and (Glc6S)6-Glc (1S,6S) for compound 2.

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高分辨率 MALDI-TOF-MS 和 MS/MS：在硫酸化寡糖结构表征中的应用

硫酸化寡糖参与重要的生物事件，这些事件通常受特定序列和硫酸化模式的调节，但其结构分析仍然具有挑战性。三种不同的硫酸化寡糖（磺达肝素、八硫酸化五糖、二硫酸化肝素衍生四糖 1 和八硫酸化麦芽七糖）2 的基质辅助激光解吸/电离质谱 (MALDI-MS) 分析使用 2-(4 -羟基苯基偶氮）苯甲酸-四甲基胍（HABA-TMG2）基质。观察到的主要离子的高分辨率质谱是成功的，并辅以串联质谱 (MS/MS) 分析进行结构评估。尽管硫酸盐损失，