Strawberry is often subjected to cold stress in temperate regions when insulation measures are not strictly applied in protected cultivation. Cold stress adversely influences plant growth and development by triggering a massive change to the transcriptome. To provide the potential strategies in improving strawberry cold tolerance and give a glimpse into the understanding of the complex cold signaling pathways in plants, this study identified attractive candidate genes and revealed diverse regulatory networks that responded to cold stress in strawberry (Fragaria×ananassa) by a transcriptomic analysis. Totally, there were 2397 differentially expressed genes (DEGs) under cold stress treatment (T1) vs. normal treatment (CK). Of these, 1180 DEGs were upregulated, while 1217 DEGs were downregulated. Functional enrichment analysis showed that DEGs were significantly (adjusted value < 0.05) overrepresented in six pathways including plant hormone signal transduction, flavonoid biosynthesis, mitogen-activated protein kinase (MAPK) signaling, starch and sucrose metabolism, circadian rhythm, and alpha-linolenic acid metabolism. The cold signaling initiated expression of downstream cold-responsive (COR) genes with cis-acting element ABRE or CRT/DRE in the ABA-independent or ABA-dependent pathway to impel plant defense against the stress. Strikingly, GIGANTEA (gene id 101308922), two-component response regulator-like PRR95 (gene id 101295449), and ethylene-responsive transcription factor ERF105-like (gene id 101295082) were dramatically induced under low-temperature treatment, indicating that they played an important role in response to cold stress in strawberry.

中文翻译：

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转录组分析揭示了响应草莓冷胁迫（Fragaria×ananassa）的多种调控网络。

如果在保护性栽培中未严格采取保温措施，草莓通常会在温带地区遭受冷胁迫。冷胁迫通过触发转录组的巨大变化，对植物的生长和发育产生不利影响。为了提供改善草莓耐寒性的潜在策略并了解植物中复杂的寒冷信号传导途径，本研究鉴定了有吸引力的候选基因并揭示了多种调控网络，它们通过以下方式响应草莓的低温胁迫（Fragaria×ananassa）转录组分析。总共有2397个差异表达基因（DEG）在冷胁迫处理（T1）与正常治疗（CK）。其中，上调了1180个DEG，而下调了1217个DEG。功能富集分析表明DEGs显着（调整后值<0.05）在植物激素信号转导，类黄酮生物合成，促分裂原活化蛋白激酶（MAPK）信号传导，淀粉和蔗糖代谢，昼夜节律和α-亚麻酸代谢等六个途径中均过分表达。冷信号启动了顺式作用元件ABRE或CRT / DRE在ABA非依赖性或ABA依赖性途径中下游冷应答（COR）基因的表达，以促进植物对逆境的防御。引人注目的是，在低温处理下会显着诱导出GIGANTEA（基因ID 101308922），类似两组分的反应调节剂PRR95（基因ID 101295449）和乙烯响应转录因子ERF105样（基因ID 101295082），表明它们发挥了作用。草莓在应对冷胁迫中起重要作用。