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A rapid and simple chemical method for the preparation of Ag colloids for surface-enhanced Raman spectroscopy using the Ag mirror reaction
Vibrational Spectroscopy ( IF 2.5 ) Pub Date : 2018-09-01 , DOI: 10.1016/j.vibspec.2018.06.011
Rajapandiyan Panneerselvam 1 , Li Xiao 2 , Ken B Waites 3 , T Prescott Atkinson 4 , Richard A Dluhy 1
Affiliation  

Colloidal silver (Ag) nanoparticles (AgNP) have been widely used for surface-enhanced Raman spectroscopy (SERS) applications. We report a simple, rapid and effective method to prepare AgNP colloids for SERS using the classic organic chemistry Ag mirror reaction with Tollens' reagent. The AgNP colloid prepared with this process was characterized using SEM, and the reaction conditions further optimized using SERS measurements. It was found that Ag mirror reaction conditions that included 20 mM AgNO3, 5 min reaction time, and 0.5 M glucose produced AgNP colloids with an average size of 319.1 nm (s.d ±128.1). These AgNP colloids exhibited a significant SERS response when adenine was used as the reporter molecule. The usefulness of these new AgNP colloids was demonstrated by detecting the nucleotides adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), cytidine 5'-monophosphate (CMP), and uridine 5'-monophosphate (UMP). A detection limit of 500 nM for AMP was achieved with the as-prepared AgNP colloid. The bacterium Mycoplasma pneumoniae was also easily detected in laboratory culture with these SERS substrates. These findings attest to the applicability of this AgNP colloid for the sensitive and specific detection of both small biomolecules and microorganisms.

中文翻译:

一种使用银镜反应制备用于表面增强拉曼光谱的银胶体的快速简便的化学方法

胶体银 (Ag) 纳米粒子 (AgNP) 已广泛用于表面增强拉曼光谱 (SERS) 应用。我们报告了一种使用经典有机化学银镜反应与 Tollens 试剂制备用于 SERS 的 AgNP 胶体的简单、快速和有效的方法。使用该工艺制备的 AgNP 胶体使用 SEM 进行表征,并使用 SERS 测量进一步优化反应条件。发现包括 20 mM AgNO3、5 分钟反应时间和 0.5 M 葡萄糖的 Ag 镜像反应条件产生的 AgNP 胶体的平均尺寸为 319.1 nm (sd ±128.1)。当腺嘌呤用作报告分子时,这些 AgNP 胶体表现出显着的 SERS 响应。通过检测核苷酸腺苷 5'-单磷酸 (AMP),证明了这些新的 AgNP 胶体的有用性,鸟苷 5'-单磷酸 (GMP)、胞苷 5'-单磷酸 (CMP) 和尿苷 5'-单磷酸 (UMP)。使用制备的 AgNP 胶体实现了 500 nM 的 AMP 检测限。使用这些 SERS 底物在实验室培养物中也很容易检测到肺炎支原体。这些发现证明了这种 AgNP 胶体对于小生物分子和微生物的灵敏和特异性检测的适用性。
更新日期:2018-09-01
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