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Laser interferometry of the hydrolytic changes in protein solutions: the refractive index and hydration shells
Journal of Biological Physics ( IF 1.8 ) Pub Date : 2018-05-11 , DOI: 10.1007/s10867-018-9494-7
R M Sarimov 1 , T A Matveyeva 1 , V N Binhi 1, 2
Affiliation  

Using an original laser interferometer of enhanced sensitivity, an increase in the refractive index of a protein solution was observed during the reaction of proteolysis catalyzed by pepsin. The increase in the refractive index of the protein solution at a concentration of 4 mg/ml was 9×10−6$ 9 \times 10^{-6} $ for bovine serum albumin and 2.4×10−6$2.4 \times 10^{- 6}$ for lysozyme. The observed effect disproves the existing idea that the refractive index of protein solutions is determined only by their amino acid composition and concentration. It is shown that the refractive index also depends on the state of protein fragmentation. A mathematical model of proteolysis and a real-time method for estimating the state of protein hydration based on the measurement of refractive index during the reaction are proposed. A good agreement between the experimental and calculated time dependences of the refractive index shows that the growth of the surface of protein fragments and the change in the number of hydration cavities during proteolysis can be responsible for the observed effect.

中文翻译:

蛋白质溶液水解变化的激光干涉测量:折射率和水合壳

使用具有增强灵敏度的原始激光干涉仪,在胃蛋白酶催化的蛋白水解反应期间观察到蛋白质溶液的折射率增加。对于牛血清白蛋白,4mg/ml 浓度的蛋白质溶液的折射率增加为 9×10−6$ 9 \times 10^{-6} $ 和 2.4×10−6$2.4 \times 10^ {- 6}$ 为溶菌酶。观察到的效果反驳了现有的观点,即蛋白质溶液的折射率仅由其氨基酸组成和浓度决定。结果表明,折射率还取决于蛋白质碎裂的状态。提出了蛋白质水解的数学模型和基于反应过程中折射率测量来估计蛋白质水合状态的实时方法。
更新日期:2018-05-11
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