Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10–30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were 50 °C and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.

从Trametes polyzona中纯化了两种锰过氧化物酶（MnPs）和MnP1和MnP2，以及一种漆酶Lac1。KU-RNW027。两种MnP在有机溶剂中均显示出高稳定性，从而触发了它们的活性。金属离子可以一定浓度激活两种MnP。在无氧化还原介质的系统中，两种MnP和Lac1在染料降解和药物产品失活中起重要作用。他们在10–30分钟内完全降解了Remazol亮蓝色（25 mg / L），并显示出对Remazol海军蓝和Remazol亮黄色的高降解活性，而Lac1可以去除75％的Remazol红。这三种纯化的酶有效地使四环素，强力霉素，阿莫西林和环丙沙星失活。最佳反应条件为50°C和pH 4.5。两种MnP被有机溶剂和金属离子激活，表明使用T. polyzona的功效 KU-RNW027用于在有机溶剂和金属离子污染的环境中对芳香族化合物进行生物修复，而无需氧化还原介体补充剂。