Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of P. eryngii KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An A4-linked sequence was identified and used to find the adjacent genomic region with the entire motif of the A locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker 7-2₂₉₉ distinguished A4 mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the A4 locus had a general feature consisting of the putative HD1 and HD2 genes. Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. The ACAAT motif, which was reported to have relevance to sex determination, was found in the intergenic region. The SCAR marker could be applicable in the classification of mating types in the P. eryngii breeding program, and the A4 locus could be the basis for a multi-allele detection marker.

A和B交配型位点的等位基因差异是杏鲍菇属交配发展的先决条件。因此，杂交受到近亲繁殖中的这种生物屏障的阻碍。利用随机扩增的多态性DNA来研究与杏鲍菇KNR2312交配类型相关的分子标记，以提高杂交效率。的A4 -连接的序列被确定，并用于与所述的整个基序以找到相邻的基因组区域甲轨迹从由PacBio测序重叠群。序列鉴定的扩增区域标记7-2 ₂₉₉杰出的A4KNR2312和其他菌株的交配型单核体。对侧翼序列的BLAST搜索显示，A4基因座具有由假定的HD1和HD2基因组成的一般特征。两个推定的HD转录因子均包含一个同源结构域序列和一个核定位序列。但是，仅在HD1蛋白中发现有效的二聚化基序。据报道，在基因间区域发现了与性别决定有关的ACAAT基序。SCAR标记可能适用于P. eryngii繁殖计划中的交配类型分类，而A4位点可能是多等位基因检测标记的基础。