Recent studies have reported elevated plasma homocysteine and reduced folate and vitamin B levels in patients with multiple sclerosis (MS). In this study, we aimed to investigate the association between MS and the following four DNA polymorphisms: MTR A[2756]G, MTHFR C[677]T, MTHFR A[1298]C and MTRR A[66]G. The DNA polymorphisms were genotyped in 80 patients with confirmed MS and 80 healthy control age- and gender-matched subjects using PCR-RFLP approach. Our results show that the frequency of the T/T genotype homozygotes for the MTHFR C[677]T polymorphism was significantly higher in patients than in controls (p = 0.04, OR: 3.16, 95% CI: 1.23-8.17). In turn, the A/A genotype of the MTHFR A[1298]C polymorphism was more frequent in controls than in patients (41.3% vs. 32.5%, p = 0.04). There were no differences in distribution of genotypes for the MTR A[66]G and MTR A[2756]C polymorphisms between patients with MS and controls (p > 0.05). Our findings suggested that the MTHFR C[677]T and MTHFR A[1298]C gene polymorphisms might be associated with MS as genetic factors influencing the risk of the disease.

中文翻译：

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遗传多态性MTHFR（C677T，A1298C），MTR（A2756G）和MTRR（A66G）基因与多发性硬化之间的关系：病例对照研究。

最近的研究报道，多发性硬化症（MS）患者血浆高半胱氨酸水平升高，叶酸和维生素B水平降低。在这项研究中，我们旨在研究MS与以下四个DNA多态性之间的关联：MTR A [2756] G，MTHFR C [677] T，MTHFR A [1298] C和MTRR A [66] G。使用PCR-RFLP方法对80例确诊MS患者和80例健康对照年龄和性别匹配的受试者进行DNA多态性基因分型。我们的结果表明，患者中MTHFR C [677] T多态性的T / T基因型纯合子频率显着高于对照组（p = 0.04，OR：3.16，95％CI：1.23-8.17）。反过来，MTHFR A [1298] C多态性的A / A基因型在对照组中比在患者中更为常见（41.3％对32.5％，p = 0.04）。MS患者和对照组之间MTR A [66] G和MTR A [2756] C多态性的基因型分布没有差异（p> 0.05）。我们的研究结果表明，MTHFR C [677] T和MTHFR A [1298] C基因多态性可能与MS相关，因为遗传因素影响该疾病的风险。