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Pulsed-field gel electrophoresis fingerprinting of Campylobacter jejuni and Campylobacter coli strains isolated from clinical specimens, Iran.
International Microbiology ( IF 3.1 ) Pub Date : 2019-03-14 , DOI: 10.1007/s10123-019-00062-8
Mahdi Ghorbanalizadgan 1 , Bita Bakhshi 1 , Saeed Shams 2 , Shahin Najar-Peerayeh 1
Affiliation  

The aim of this study was to determine the clonal correlation of Campylobacter strains isolated from diarrheal children under 5 years of age in Iran using the PFGE method and to determine the antimicrobial susceptibility and virulence gene content of strains. Of 750 patients with bacterial diarrhea, 33 (4%) Campylobacter spp., including 31 C. jejuni (94%) and 2 C. coli (6%), were isolated during 18-month period in Tehran, Iran. Except for one strain, remaining Campylobacter strains were positive for the flaA gene. A complete set of cytolethal distending toxin (CDT) encoding genes (cdtABC) were detected in 52% of the C. jejuni strains, while the 2 C. coli isolates under study only harbored cdtA and cdtB of the CDT cluster. All strains were resistant to at least three antibiotic classes. Resistance to ampicillin among C. coli and C. jejuni strains was 100% and 84%, respectively, and 80% of all strains were susceptible to gentamicin. PFGE genotyping generated 19 pulsotypes with two major clusters, displaying the maximum and minimum similarity of 100% and 26%, respectively. The C. coli strains showed clearly distinct pulsotypes and each fell within separate clusters. A very homogeneous Campylobacter population was detected among Iranian patients with 33 % of strains showing identical banding patterns and no clear correlation was observed between antibiotic resistance profiles and PFGE patterns of the isolates.

中文翻译:

从伊朗临床标本中分离出的空肠弯曲杆菌和大肠杆菌弯曲杆菌的脉冲场凝胶电泳指纹图谱。

这项研究的目的是使用PFGE方法确定从伊朗5岁以下腹泻儿童中分离出的弯曲杆菌菌株的克隆相关性,并确定该菌株的抗菌敏感性和致病性基因含量。在伊朗德黑兰的18个月中,分离出750例细菌性腹泻患者,其中33例(4%)弯曲杆菌属,包括空肠弯曲杆菌31例(94%)和2大肠杆菌(6%)。除一种菌株外,其余弯曲杆菌菌株对fla A基因呈阳性。在52%的空肠弯曲菌中检测到完整的细胞致死性扩张毒素(CDT)编码基因(cdt ABC)菌株,而正在研究的2个大肠杆菌分离株仅包含CDT簇的cdt A和cdtB。所有菌株均对至少三种抗生素类别具有抗性。大肠杆菌空肠弯曲杆菌菌株对氨苄西林的耐药性分别为100%和84%,所有菌株中的80%对庆大霉素敏感。PFGE基因分型产生了带有两个主要簇的19个脉冲型,分别显示出最大和最小相似度分别为100%和26%。在大肠菌株清楚地表明不同pulsotypes并且每个下降独立的集群内。非常均匀的弯曲杆菌 在伊朗患者中检测到了该人群,其中33%的菌株显示出相同的条带模式,并且在分离菌株的抗生素耐药性谱图和PFGE模式之间未观察到明显的相关性。
更新日期:2019-03-14
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