Molecular Imaging ( IF 2.8 ) Pub Date : 2019-01-04 , DOI: 10.1177/1536012118821032 Jing Zhao 1, 2, 3 , Zhanwen Zhang 3, 4, 5 , Dahong Nie 3, 6 , Hui Ma 3, 4 , Gongjun Yuan 3, 4 , Shu Su 3, 4 , Shaoyu Liu 3, 4 , Sheng Liu 1, 2 , Ganghua Tang 3, 4
Objective:
To evaluate the preclinical value of 18F-fluoropropionic acid (18F-FPA) and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) for imaging HCCs.
Methods:
The 18F-FPA and 18F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The 18F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The 18F-FPA PET imaging was performed in different tumor animal models and compared with 18F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines.
Results:
In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of 18F-FPA. The tumor-to-liver ratio of 18F-FPA was superior to that of 18F-FDG in the SK-Hep1 and HepG2 tumors (P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of 18F-FDG was higher than 18F-FPA (P < .01). FASN was highly expressed in cell lines with high 18F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high 18F-FDG uptake. The 18F-FPA uptake correlated with FASN (r = 0.89, P = .014) and MMP2 (r = 0.77, P = .002) expressions.
Conclusions:
PET imaging with 18F-FPA combined with 18F-FDG can be an alternative for detecting HCC.
中文翻译:
肝细胞癌的PET成像:18F-氟丙酸作为18F-氟脱氧葡萄糖的放射性示踪剂。
目的:
为了评估18 F-氟丙酸(18 F-FPA)和18 F-氟脱氧葡萄糖(18 F-FDG)正电子发射断层扫描(PET)的临床前价值,以对HCC成像。
方法:
的18 F-FPA和18在3 HCC细胞系(Hep3B细胞,HepG2细胞和SK-HEP1)F-FDG摄取模式在体外和体内进行了评估。在18使用抑制实验用奥利司他和5-十四烷氧基-2-糠酸F-FPA摄取机理。该18在不同肿瘤的动物模型中进行F-FPA的PET成像和相比18 F-FDG。我们还评估了这些细胞系中葡萄糖转运蛋白1(GLUT1),脂肪酸合酶(FASN)和基质金属蛋白酶2(MMP2)的表达。
结果:
体外实验表明,放射性示踪剂的摄取模式在HCC细胞系中是互补的。奥利司他和5-十四烷氧基-2-糠酸减少了18 F-FPA的吸收。在SK-Hep1和HepG2肿瘤中,18 F-FPA的肝癌比率优于18 F-FDG(P <.05)。但是,在Hep3B肿瘤中,肝-肝正常摄取的18 F-FDG高于18 F-FPA(P <.01)。FASN在高18 F-FPA摄取的细胞系中高表达,而GLUT1在18 F-FDG高摄取的细胞系中高表达。在18F-FPA摄取与FASN(r = 0.89,P = .014)和MMP2(r = 0.77,P = .002)表达相关。
结论:
将18 F-FPA与18 F-FDG结合使用的PET成像可以替代检测HCC。