SummaryEndometrial injury is an important cause of intrauterine adhesion (IUA), amenorrhea and infertility in women, with limited effective therapies. Recently, stem cells have been used in animal experiments to repair and improve injured endometrium. To date, our understanding of adipose-derived stem cells (ADSCs) in endometrial injury repair and their further therapeutic mechanisms is incomplete. Here, we examined the benefit of ADSCs in restoration of injured endometrium by applying a rat endometrial injury model. The results revealed by immunofluorescence showed that green fluorescent protein (GFP)-labelled ADSCs can differentiate into endometrial epithelial cells in vivo. At 30 days after ADSCs transplantation, injured endometrium was significantly improved, with increased microvessel density, endometrial thickness and glands when compared with the model group. Furthermore, the fertility of rats with injured endometrium in ADSCs group was improved and had a higher conception rate (60% vs 20%, P = 0.014) compared with the control phosphate-buffered saline (PBS) group. However, there was no difference in the control group compared with the sham group. In addition, expression levels of the oestrogen receptor Eα/β (ERα, ERβ) and progesterone receptor (PR) detected by western blot and enzyme-linked immunosorbent assay (ELISA) were higher in the ADSCs group than in the PBS group. Taken together, these results suggested that ADSC transplantation could improve endometrial injury as a novel therapy for IUA.

中文翻译：

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脂肪干细胞移植改善子宫内膜损伤修复

摘要子宫内膜损伤是导致女性宫腔粘连（IUA）、闭经和不孕的重要原因，有效的治疗方法有限。最近，干细胞已被用于动物实验以修复和改善受损的子宫内膜。迄今为止，我们对子宫内膜损伤修复中的脂肪干细胞（ADSCs）及其进一步治疗机制的理解还不完整。在这里，我们通过应用大鼠子宫内膜损伤模型检查了 ADSC 在修复受损子宫内膜中的益处。免疫荧光显示的结果表明，绿色荧光蛋白（GFP）标记的ADSCs可以分化为子宫内膜上皮细胞体内. ADSCs移植后30天，与模型组相比，损伤的子宫内膜明显改善，微血管密度、子宫内膜厚度和腺体增加。此外，ADSCs组子宫内膜损伤大鼠的生育能力得到改善，受孕率更高（60% vs 20%，磷= 0.014) 与对照磷酸盐缓冲盐水 (PBS) 组相比。然而，与假手术组相比，对照组没有差异。此外，通过蛋白质印迹和酶联免疫吸附试验（ELISA）检测的雌激素受体Eα/β（ERα，ERβ）和孕激素受体（PR）在ADSCs组中的表达水平高于PBS组。总之，这些结果表明 ADSC 移植可以改善子宫内膜损伤，作为 IUA 的一种新疗法。