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The combination of Astragalus membranaceus extract and ligustrazine to improve the inflammation in rats with thrombolytic cerebral ischemia.
International Journal of Immunopathology and Pharmacology ( IF 3.5 ) Pub Date : 2019-08-15 , DOI: 10.1177/2058738419869055
Ruihuan Pan 1 , Mingchao Zhou 2 , Yiping Zhong 3 , Jingping Xie 4 , Shanshan Ling 1, 3 , Xialin Tang 3 , Yan Huang 5 , Hongxia Chen 1
Affiliation  

The purpose of the study was to evaluate the effect of Astragalus membranaceus extract and ligustrazine combination on ameliorating inflammation in cerebral ischemic rats that have undergone thrombolysis. Astragalus membranaceus and ligustrazine per se, or a combination of A. membranaceus and ligustrazine was administered by intraperitoneal injection immediately after surgery and sham surgery. After the induction of thrombolysis, the neurological function was measured and cerebral lesion volume was determined. The regulatory T cells in the spleen were measured by flow cytometry. To explore the protective effects of the combination drug on the neurological function and inflammation, the expression of transcription factor Foxp3 and cytokines, including transforming growth factor beta 1, interleukin 10, interleukin 4, interleukin 1 beta, interferon gamma, interleukin 17, in damaged brain was examined using reverse transcription polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay. The cerebral lesion volume was markedly reduced in the combination drug-treated rats compared to the rats treated with either A. membranaceus or ligustrazine alone (P < 0.05). The neurological function, regulatory T cells, transcription factor Foxp3, transforming growth factor beta 1, interleukin 10, and interleukin 4 were markedly elevated in the rats treated with combination drugs (P < 0.05). The expression of interleukin 1 beta, interferon gamma, and interleukin 17 was reduced in the rats treated with combination drug therapy (P < 0.05). Treatment with a combination of A. membranaceus and ligustrazine can ameliorate inflammation after thrombolysis and regulate the related cytokines by elevating the expression of endogenous regulatory T cells.

中文翻译:

黄芪提取物与川gust嗪联用改善溶栓性脑缺血大鼠的炎症。

这项研究的目的是评估黄芪提取物和川gust嗪组合对缓解溶栓后脑缺血大鼠炎症的作用。手术和假手术后立即通过腹膜内注射施用黄芪膜和川gust嗪,或膜曲霉和川gust嗪的组合。溶栓后,测定神经功能,测定脑病变量。通过流式细胞术测量脾脏中的调节性T细胞。为了探讨联合用药对神经功能和炎症的保护作用,我们研究了转录因子Foxp3和细胞因子的表达,包括转化生长因子β1,白介素10,白介素4,白介素1 beta,使用逆转录聚合酶链反应,蛋白质印迹和酶联免疫吸附试验检测受损脑中的干扰素γ,白介素17。与仅用膜曲霉或川gust嗪治疗的大鼠相比,在用药物联合治疗的大鼠中脑病变体积明显减少(P <0.05)。联合用药组大鼠神经功能,调节性T细胞,转录因子Foxp3,转化生长因子β1,白介素10和白介素4明显升高(P <0.05)。在联合药物治疗的大鼠中,白细胞介素1β,干扰素γ和白介素17的表达降低(P <0.05)。用A组合治疗。
更新日期:2019-11-01
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