The use of tandem mass spectrometry to identify and characterize sites of protein adenosine diphosphate (ADP) ribosylation will be reviewed. Specifically, we will focus on data acquisition schemes and fragmentation techniques that provide peptide sequence and modification site information. Also discussed are uses of synthetic standards to aid characterization, and an enzymatic method that converts ADP-ribosylated peptides into ribosyl mono phosphorylated peptides making identification amenable to traditional phosphopeptide characterization methods. Finally the potential uses of these techniques to characterize poly ADP-ribosylation sites, and inherent challenges, are addressed.

中文翻译：

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二磷酸腺苷核糖基化肽的串联质谱表征综述

将审查使用串联质谱法来鉴定和表征蛋白质二磷酸腺苷 (ADP) 核糖基化位点。具体来说，我们将专注于提供肽序列和修饰位点信息的数据采集方案和片段化技术。还讨论了使用合成标准来帮助表征，以及一种将 ADP 核糖基化肽转化为核糖基单磷酸化肽的酶促方法，使鉴定适合传统的磷酸肽表征方法。最后，解决了这些技术在表征聚 ADP 核糖基化位点的潜在用途和固有挑战。