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Identification and mapping of a putative stress response regulator gene in barley.
Plant Molecular Biology ( IF 5.1 ) Pub Date : 2002-07-26 , DOI: 10.1023/a:1016051332488
Marina Malatrasi 1 , Timothy J Close , Nelson Marmiroli
Affiliation  

Plants respond to environmental stress with a number of physiological and developmental changes. Water deficit is one of the major factors limiting plant growth and development and crop productivity. One response of plants to water deficit is accumulation of abscisic acid (ABA). An increase of ABA is responsible for the induction of many genes, presumably some of which contribute to drought tolerance. Analysis of gene expression in barley seedling shoots by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) led to the isolation of several drought-, cold- and ABA-induced partial cDNA fragments. Here we extensively characterize one of these cDNAs, designated DD6. First, a larger cDNA was extended from DD6 by 5'-RACE (rapid amplification of cDNA ends). Subsequently, the corresponding gene was isolated by screening a barley BAC library, and the sequences of the transcribed and flanking regions were determined. The deduced amino acid sequence has similarity to an Arabidopsis hypothetical protein and to a human and mouse DNA-binding protein. The corresponding gene, named Srg6 (stress-responsive gene), was mapped in a barley doubled haploid mapping population to chromosome 7H between markers ABC455 and salfp76, within a region that previously has been linked to osmotic adaptation in barley and other grass genomes.

中文翻译:

大麦中假定的应激反应调节基因的鉴定和作图。

植物通过许多生理和发育变化来应对环境胁迫。缺水是限制植物生长发育和作物生产力的主要因素之一。植物对缺水的一种反应是脱落酸(ABA)的积累。ABA的增加导致许多基因的诱导,大概其中一些有助于干旱耐受。大麦幼苗芽中基因表达的差异显示逆转录聚合酶链反应(DDRT-PCR)分析导致了几个干旱,寒冷和ABA诱导的部分cDNA片段的分离。在这里,我们广泛地表征了这些cDNA之一,命名为DD6。首先,更大的cDNA通过5'-RACE(快速扩增cDNA末端)从DD6延伸出来。后来,通过筛选大麦BAC文库分离相应的基因,并确定转录和侧翼区域的序列。推导的氨基酸序列与拟南芥假说蛋白以及人和小鼠DNA结合蛋白具有相似性。相应的基因名为Srg6(应激反应基因),已在一个大麦加倍单倍体作图群体中定位到标记ABC455和salfp76之间的7H染色体上,该区域以前与大麦和其他草基因组的渗透适应有关。
更新日期:2019-11-01
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