Thinopyrum ponticum (Podpěra, 1902) Z.-W. Liu & R.-C.Wang, 1993 is an important polyploid wild perennial Triticeae species that is widely used as a source of valuable genes for wheat but its genomic constitution has long been debated. For its chromosome identification, only a limited set of FISH probes has been used. The development of new cytogenetic markers for Th. ponticum chromosomes is of great importance both for cytogenetic characterization of wheat-wheatgrass hybrids and for fundamental comparative studies of phylogenetic relationships between species. Here, we report on the development of five cytogenetic markers for Th. ponticum based on repetitive satellite DNA of which sequences were selected from the whole genome sequence of Aegilops tauschii Cosson, 1849. Using real-time quantitative PCR we estimated the abundance of the found repeats: P720 and P427 had the highest abundance and P132, P332 and P170 had lower quantity in Th. ponticum genome. Using fluorescence in situ hybridization (FISH) we localized five repeats to different regions of the chromosomes of Th. ponticum. Using reprobing multicolor FISH we colocalized the probes between each other. The distribution of these found repeats in the Triticeae genomes and its usability as cytogenetic markers for chromosomes of Th. ponticum are discussed.

中文翻译：

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ponticum（Podp。）Z.-W.的新细胞遗传学标记的开发。Liu＆R.-C. 王

ponticum ponyumum（Podpěra，1902）Liu＆R.-C. Wang，1993年，是一种重要的多倍体野生多年生黑麦草种，被广泛用作小麦有价值的基因来源，但其基因组构成一直存在争议。对于其染色体鉴定，仅使用了有限的一组FISH探针。Th的新细胞遗传标记的发展。ponticum染色体对于小麦-小麦草杂种的细胞遗传学表征以及物种间系统发生关系的基础比较研究都非常重要。在这里，我们报告Th的五个细胞遗传标记的发展。基于重复卫星DNA的ponticum，其序列选自Aegilops tauschii Cosson的全基因组序列，1849年。使用实时定量PCR，我们估计了发现的重复序列的丰度：P720和P427的丰度最高，而P132，P332和P170的Th含量较低。ponticum基因组。使用荧光原位杂交（FISH），我们将5个重复序列定位到Th染色体的不同区域。ponticum。使用多色FISH探测，我们将探针彼此共定位。这些发现的重复序列在小麦的基因组中的分布及其作为Th染色体细胞遗传标记的可用性。ponticum进行了讨论。使用多色FISH探测，我们将探针彼此共定位。这些发现的重复序列在小麦的基因组中的分布及其作为Th染色体细胞遗传标记的可用性。ponticum进行了讨论。使用多色FISH探测，我们将探针彼此共定位。这些发现的重复序列在小麦的基因组中的分布及其作为Th染色体细胞遗传标记的可用性。ponticum进行了讨论。