Objective: To evaluate the expression levels of adipokines in the synovium and infrapatellar fat pad (IPFP) in osteoarthritis (OA) patients with and without metabolic syndrome (MetS). Methods: 120 female patients with OA were enrolled, and 60 healthy women matched body mass index, age, and sex, served as controls. Adipokines levels were measured using a sandwich enzyme-linked immunosorbent assay of the serum of all participants and synovial fluid (SF) of OA patients. Local expression levels of adipokines in the synovium and IPFP were examined by immunohistochemical analysis. The amount of adipokine proteins was analyzed using Western blot, and adipokine mRNA expressions were determined via quantitative real-time polymerase chain reaction (qRT-PCR). Results: Serum leptin levels were significantly higher in the non-MetS-OA group than those in controls (7.97 vs. 4.24 ng/ml, p< 0.001), and even higher leptin levels were found in the MetS-OA group (19.05 ng/ml; p< 0.001 for both). Serum adiponectin levels were significantly lower in the MetS-OA group than those in controls (8.09 vs. 10.07 μg/ml, respectively; p= 0.001). The synovium and IPFP in the MetS-OA group secreted more leptin and less adiponectin than those in the non-MetS-OA group (Leptin: 5.32 vs. 1.28 in synovium, respectively; p= 0.028; 6.44 vs. 0.88 in IPFP, respectively; p= 0.017. Adiponectin: 1.12 vs. 0.12 in synovium, respectively; p= 0.042; 1.07 vs. 0.09 in IPFP, respectively; p= 0.027). Resistin expression levels in the serum, SF, and articular tissues were similar among the groups. Conclusions: Expressions of adipokines were different in the synovium and IPFP of OA patients with and without MetS.

中文翻译：

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伴和不伴代谢综合征的骨关节炎患者滑膜和in下脂肪垫中脂肪因子的差异表达。

目的：评估伴或不伴代谢综合征（MetS）的骨关节炎（OA）患者滑膜和pat下脂肪垫（IPFP）中脂肪因子的表达水平。方法：纳入120例女性OA患者，以体重指数，年龄和性别相匹配的60例健康女性作为对照。使用夹心酶联免疫吸附法对OA患者所有参与者的血清和滑液（SF）进行脂肪因子水平测定。通过免疫组织化学分析检查滑膜和IPFP中脂肪因子的局部表达水平。使用蛋白质印迹分析脂肪因子蛋白的量，并通过定量实时聚合酶链反应（qRT-PCR）确定脂肪因子mRNA的表达。结果：非MetS-OA组的血清瘦素水平显着高于对照组（7.97比4.24 ng / ml，p <0.001），MetS-OA组的瘦素水平更高（19.05 ng / ml）。 ;两者均<0.001）。MetS-OA组的血清脂联素水平显着低于对照组（分别为8.09和10.07μg/ ml； p = 0.001）。与非MetS-OA组相比，MetS-OA组的滑膜和IPFP分泌更多的瘦素和脂联素（滑膜中的Leptin分别为5.32和1.28； pFP分别为p = 0.028、6.44和0.88）。 ；脂联素：滑膜中分别为1.12和0.12； p = 0.017； IPFP中分别为p = 0.042、1.07和0.09； p = 0.027）。各组之间血清，SF和关节组织中的抵抗素表达水平相似。结论：