Fibroblast growth factor 2 (FGF2) regulates the wound repair process and it is secreted by inflammatory and endothelial cells, and by myofibroblasts. This study aimed to establish the expression patterns of FGF2 and myofibroblastic differentiation during wound healing in rats treated with subcutaneous ozone injection. We created full-thickness excisional wounds in rats, and the healing process was analyzed through morphometric analyses and digital quantification of immunoreactivity of smooth muscle actin and FGF2. Ozone therapy-treated wounds presented granulation tissue with a reduced number of inflammatory cells and greater dermal cellularity, and intense collagen deposition. FGF2 immunoreactivity, microvessel density, and amount of myofibroblasts were significantly higher in treated wounds compared to controls. In conclusion, it was demonstrated that subcutaneous injections of ozone accelerate and ameliorate wound repairing process. Moreover, injectable ozone therapy's action mechanism may be associated with FGF2 overexpression.

中文翻译：

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皮下注射臭氧对大鼠伤口愈合的影响。

成纤维细胞生长因子2（FGF2）调节伤口修复过程，并由炎症细胞和内皮细胞以及成肌纤维细胞分泌。这项研究旨在建立皮下注入臭氧的大鼠伤口愈合过程中FGF2的表达模式和肌成纤维细胞分化。我们在大鼠中创建了全层切除伤口，并通过形态计量学分析和平滑肌肌动蛋白和FGF2免疫反应性的数字定量分析了愈合过程。臭氧疗法治疗的伤口呈现出的肉芽组织中炎性细胞数量减少，真皮细胞增多，胶原沉积强烈。与对照组相比，治疗伤口的FGF2免疫反应性，微血管密度和成肌纤维细胞数量明显更高。结论，事实证明，皮下注射臭氧可加速并改善伤口修复过程。此外，可注射臭氧疗法的作用机制可能与FGF2过表达有关。