We investigated the potential nephroprotective effects of misoprostol (MP) on doxorubicin (DOX) induced renal injury using histologic and biochemical assessment of rat kidneys. We used 21 male rats divided into three groups: group 1, control; group 2, DOX; group 3, DOX + MP. The control group was given 0.5 ml 0.9% NaCl and 1 ml 0.9% NaCl orally for 6 days. DOX was administered as a single dose of 20 mg/kg on day 3. MP was administered orally for 6 days. We found that treatment with MP decreased serum creatinine and blood urea nitrogen levels significantly. DOX increased the malondialdehyde level and decreased catalase, superoxide dismutase activities and glutathione level. These alterations were prevented in renal tissues by MP. MP also decreased NADPH oxidase-4 and caspase-3 levels. In the DOX + MP group, oxidative stress was decreased, antioxidant activity was increased and histopathological changes were reduced compared to the DOX group. Renal injury caused by DOX was attenuated by MP treatment owing to the antioxidative and anti-apoptotic effects of MP.

中文翻译：

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米索前列醇对阿霉素诱导的大鼠肾损伤的影响。

我们使用大鼠肾脏的组织学和生化评估研究了米索前列醇（MP）对阿霉素（DOX）诱导的肾损伤的潜在肾脏保护作用。我们使用了21只雄性大鼠，将其分为三组：第1组，对照组；第2组。第2组，DOX；第3组，DOX + MP。对照组口服0.5 ml 0.9％NaCl和1 ml 0.9％NaCl，持续6天。在第3天以20 mg / kg的单剂量服用DOX。MP口服6天。我们发现，MP治疗可显着降低血清肌酐和血液尿素氮水平。DOX增加丙二醛水平，降低过氧化氢酶，超氧化物歧化酶活性和谷胱甘肽水平。MP预防了肾组织的这些改变。MP还降低了NADPH氧化酶4和caspase-3的水平。在DOX + MP组中，氧化应激降低，与DOX组相比，抗氧化剂活性增加，组织病理学改变减少。由于MP的抗氧化和抗凋亡作用，MP处理可减轻DOX引起的肾损伤。