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Profiling of microbiota in liquid baby formula consumed with an artificial nipple.
Biomedical Research ( IF 1.2 ) Pub Date : 2019-01-01 , DOI: 10.2220/biomedres.40.163
Hiroto Sano 1 , Anna Wakui 1 , Miho Kawachi 1 , Rito Kato 1 , Sachie Moriyama 1 , Mayumi Nishikata 1 , Jumpei Washio 2 , Yuki Abiko 2 , Gen Mayanagi 2 , Keiko Yamaki 3 , Reiko Sakashita 4 , Junko Tomida 5 , Yoshiaki Kawamura 5 , Kaori Tanaka 6 , Nobuhiro Takahashi 2 , Takuichi Sato 1
Affiliation  

It is suspected that oral bacteria are transferred to the liquid baby formula through the artificial nipple and multiply in the bottle after feeding. In the present study, in order to understand the influence of bacteria on liquid baby formula after feeding, the transfer of oral bacteria through artificial nipples and their survival in liquid baby formula were examined immediately after drinking as well as after storage at 4°C for 3 h. Four healthy human subjects (20-23 years old) were asked to drink liquid baby formula (Aptamil®, ca. 50 mL) from baby bottles using artificial nipples. Samples of the liquid baby formula (immediately after drinking and 3 h later) were inoculated onto blood agar plates and incubated anaerobically at 37°C for 7 days. Salivary samples from each subject and 6 newborn infants were also cultured. Genomic DNA was extracted from individual colonies, and bacterial species were identified by 16S rRNA gene sequencing. The mean amounts of bacteria (CFU/mL) were (3.2 ± 3.0) ×104 and (3.4 ± 3.3) ×104 immediately after drinking and 3 h later, respectively. Streptococcus (41.6 and 40.5%), Actinomyces (24.3 and 21.5%) and Veillonella (16.2 and 11.0%) were recovered from the samples immediately after drinking and 3 h later, respectively. On the other hand, Streptococcus (38.9%), Actinomyces (17.1%), Neisseria (9.1%), Prevotella (6.9%), Rothia (6.9%) and Gemella (5.1%) were predominant in the saliva of adult subjects, and Streptococcus (65.2%), Staphylococcus (18.5%), Gemella (8.2%) and Rothia (5.4%) were predominant in the saliva of infant subjects. From these findings, oral bacteria, e.g., Streptococcus, Gemella and Rothia, were found to transfer into the liquid baby formula through artificial nipples, and the bacterial composition in the remaining liquid baby formula was found to resemble that of human saliva. The bacterial levels were similar between immediately after drinking and when stored at 4°C for 3 h, suggesting that the remaining liquid baby formula may be preserved in a refrigerator for a specified amount of time.

中文翻译:

用人工乳头消耗的液态婴儿配方奶粉中的微生物群分析。

怀疑口腔细菌会通过人工乳头转移到液态婴儿配方食品中,并在喂食后在奶瓶中繁殖。在本研究中,为了了解细菌对喂养后液态婴儿配方奶粉的影响,我们在饮用后以及在4°C的温度下储存后,检查了人工细菌通过乳头转移口腔细菌及其在液态婴儿配方奶粉中的存活率。 3小时 要求四名健康的人类受试者(20-23岁)使用人工乳头从婴儿奶瓶中喝液态婴儿配方奶粉(约50毫升)。将液态婴儿配方食品的样品(饮用后立即和3小时后)接种到血琼脂平板上,并在37°C下厌氧孵育7天。还培养了每个受试者和6个新生儿的唾液样本。从单个菌落中提取基因组DNA,并通过16S rRNA基因测序鉴定细菌种类。饮用后立即和3小时后的平均细菌量(CFU / mL)分别为(3.2±3.0)×104和(3.4±3.3)×104。饮用后立即和3小时后分别从样品中回收链球菌(41.6%和40.5%),放线菌(24.3%和21.5%)和韦永氏菌(16.2%和11.0%)。另一方面,在成年患者的唾液中,链球菌(38.9%),放线菌(17.1%),奈瑟菌(9.1%),普雷沃菌(6.9%),罗西亚(6.9%)和双歧杆菌(5.1%)占主要地位,并且婴幼儿唾液中链球菌(65.2%),葡萄球菌(18.5%),双歧杆菌(8.2%)和Rothia(5.4%)占主要地位。从这些发现中,口腔细菌,例如链球菌,双歧杆菌和Rothia,被发现通过人造乳头转移到液态婴儿配方食品中,并且发现剩余的液态婴儿配方食品中的细菌组成类似于人唾液。饮用后立即和在4°C下储存3小时之间的细菌水平相似,这表明剩余的液态婴儿配方食品可以在冰箱中保存指定的时间。
更新日期:2019-11-01
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