Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations.,Yeast

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Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations.
Yeast ( IF 2.6 ) Pub Date : 2019-05-01 , DOI: 10.1002/yea.3397
Meizhu Wang _{1,

2} , Yu Cao ₃ , Maoning Xia ₄ , Abdullah M S Al-Hatmi _{2,

5,

6} , Weizheng Ou ₇ , Yanyan Wang ₈ , Andriy A Sibirny _{9,

10} , Liang Zhao ₁ , Chenggang Zou ₁₁ , Wanqing Liao ₁₂ , Fengyan Bai ₁₃ , Xie Zhi ₁₄ , Sybren de Hoog _{2,

6} , Yingqian Kang _{1,

3}

Affiliation

Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou & Guizhou Talent Base for Microbiology and Human Health, Key Laboratory of Medical Microbiology and Parasitology of Education Department of Guizhou, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
School of Big Health, Guizhou Medical University, Guiyang, China.
Department of Clinical Laboratory, People's Hospital of Dazu District, Chongqing, China.
Ministry of Health, Directorate General of Health Services, Ibri, Oman.
Centre of Expertise in Mycology of Radboudumc/Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
Department of Clinical Lab, Guiyang Public Health Treatment Center, Guiyang, China.
Infection Control Section, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Department of Biotechnology and Microbiology, University of Rzeszow, Rzeszow, Poland.
Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine.
State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan University, Kunming, China.
Shanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Department of Dermatology, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.

A set of 185 strains of Candida albicans from patients with vulvovaginal candidiasis (VVC) and from non-VVC clinical sources in southwest China was analysed. Strains were subjected to genotyping using CAI microsatellite typing and amplification of an intron-containing region of the 25S rRNA gene. Microsatellite genotypes of strains from non-VVC sources showed high polymorphism, whereas those of VVC were dominated by few, closely similar genotypes. However, among non-VVC strains, two genotypes were particularly prevalent in patients with lung cancer. 25S rDNA genotype A was dominant in VVC sources (86.7%), whereas genotypes A, B, and C were rather evenly distributed among non-VVC sources; known genotypes D and E were not found. In an experimental mouse model, isolates from lung cancer and AIDS patients proved to have higher virulence than VVC strains. Among 156 mice infected with C. albicans, 19 developed non-invasive urothelial carcinoma. No correlation could be established between parameters of virulence, source of infection, and incidence of carcinoma. C. albicans strains from VVC were less susceptible to itraconazole than the strains from non-VVC sources, whereas there was small difference in antifungal susceptibility between different 25S rDNA genotypes of C. albicans tested against amphotericin B, itraconazole, fluconazole, and flucytosine.

更新日期：2019-11-01

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