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Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain.
Yeast ( IF 2.6 ) Pub Date : 2019-04-19 , DOI: 10.1002/yea.3394
Wilson G Morais Junior 1 , Thályta F Pacheco 1 , Débora Trichez 1 , João R M Almeida 1 , Sílvia B Gonçalves 1
Affiliation  

Xylitol is a building block for a variety of chemical commodities, besides being widely used as a sugar substitute in the food and pharmaceutical industries. The aim of this work was to develop a microbial process for xylitol production using sugarcane bagasse hydrolysate as substrate. In this context, 218 non-Saccharomyces yeast strains were screened by growth on steam-exploded sugarcane bagasse hydrolysate containing a high concentration of acetic acid (8.0 g/L). Seven new Candida tropicalis strains were selected and identified, and their ability to produce xylitol on hydrolysate at low pH (4.6) under aerobic conditions was evaluated. The most efficient strain, designated C. tropicalis JA2, was capable of producing xylitol with a yield of 0.47 g/g of consumed xylose. To improve xylitol production by C. tropicalis JA2, a series of experimental procedures were employed to optimize pH and temperature conditions, as well as nutrient source, and initial xylose and inoculum concentrations. C. tropicalis JA2 was able to produce 109.5 g/L of xylitol with a yield of 0.86 g/g of consumed xylose, and with a productivity of 2.81 g·L·h, on sugarcane bagasse hydrolysate containing 8.0 g/L acetic acid and177 g/L xylose, supplemented with 2.0 g/L yeast nitrogen base and 4.0 g/L urea. Thus, it was possible to identify a new C. tropicalis strain and to optimize the xylitol production process using sugarcane bagasse hydrolysate as a substrate. The xylitol yield on biomass hydrolysate containing a high concentration of acetic acidobtained in here is among the best reported in the literature.

中文翻译:

新近鉴定的热带假丝酵母JA2菌株在甘蔗生物质水解产物上生产木糖醇。

木糖醇不仅是食品和制药行业中的糖替代品,而且还是多种化学商品的基础。这项工作的目的是开发一种使用甘蔗渣水解物作为底物的木糖醇生产微生物工艺。在这种情况下,通过在含有高浓度乙酸(8.0 g / L)的蒸汽爆破甘蔗渣水解物上生长来筛选出218种非酿酒酵母酵母菌株。选择并鉴定了七种新的热带假丝酵母菌株,并评估了它们在有氧条件下在低pH(4.6)条件下在水解产物上产生木糖醇的能力。最有效的菌株,称为热带念珠菌JA2,能够产生木糖醇,消耗的木糖产量为0.47 g / g。为了提高热带假丝酵母JA2的木糖醇产量,采用了一系列实验程序来优化pH和温度条件,营养源以及木糖和接种物的初始浓度。在含有8.0 g / L乙酸和177的甘蔗渣蔗渣水解物上,热带假丝酵母JA2能够生产109.5 g / L的木糖醇,消耗的木糖产量为0.86 g / g,生产率为2.81 g·L·h。 g / L木糖,补充有2.0 g / L酵母氮碱和4.0 g / L尿素。因此,有可能使用甘蔗渣水解甘蔗渣作为底物,鉴定出新的热带假丝酵母菌株并优化木糖醇生产工艺。在此获得的包含高浓度乙酸的生物质水解产物上的木糖醇收率是文献中报道的最好的之一。以及营养来源以及木糖和接种物的初始浓度。在含有8.0 g / L乙酸和177的甘蔗渣蔗渣水解物上,热带假丝酵母JA2能够生产109.5 g / L的木糖醇,消耗的木糖产量为0.86 g / g,生产率为2.81 g·L·h。 g / L木糖,补充有2.0 g / L酵母氮碱和4.0 g / L尿素。因此,有可能鉴定出一个新的热带假丝酵母菌株,并以甘蔗渣蔗渣水解产物为底物来优化木糖醇的生产过程。在此获得的包含高浓度乙酸的生物质水解产物上的木糖醇收率是文献中报道的最好的之一。以及营养来源以及木糖和接种物的初始浓度。在含有8.0 g / L乙酸和177的甘蔗渣蔗渣水解物上,热带假丝酵母JA2能够生产109.5 g / L的木糖醇,消耗的木糖产量为0.86 g / g,生产率为2.81 g·L·h。 g / L木糖,补充有2.0 g / L酵母氮碱和4.0 g / L尿素。因此,有可能使用甘蔗渣水解甘蔗渣作为底物,鉴定出新的热带假丝酵母菌株并优化木糖醇生产工艺。在此获得的包含高浓度乙酸的生物质水解产物上的木糖醇收率是文献中报道的最好的之一。在81 g·L·h的条件下,在蔗糖蔗糖水解液中加入8.0 g / L乙酸和177 g / L木糖,并补充2.0 g / L酵母氮碱和4.0 g / L尿素。因此,有可能使用甘蔗渣水解甘蔗渣作为底物,鉴定出新的热带假丝酵母菌株并优化木糖醇生产工艺。在此获得的包含高浓度乙酸的生物质水解产物上的木糖醇收率是文献中报道的最好的之一。在81 g·L·h的条件下,在蔗糖蔗糖水解液中加入8.0 g / L乙酸和177 g / L木糖,并补充2.0 g / L酵母氮碱和4.0 g / L尿素。因此,有可能鉴定出一个新的热带假丝酵母菌株,并以甘蔗渣蔗渣水解产物为底物来优化木糖醇的生产过程。在此获得的包含高浓度乙酸的生物质水解产物上的木糖醇收率是文献中报道的最好的之一。
更新日期:2019-11-01
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