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Exploring the UVB-protective efficacy of melanin precursor extracted from marine imperfect fungus: Featuring characterization and application studies under in vitro conditions.
International Microbiology ( IF 3.1 ) Pub Date : 2018-05-15 , DOI: 10.1007/s10123-018-0005-2
S K Shanuja 1 , S Iswarya 1 , J Sridevi 2 , A Gnanamani 1
Affiliation  

The present study explores the production, characterization, and application of a potentially stable melanin precursor from marine imperfect non-spore-forming Aspergillus nidulans sp. strain SG 28. Growth of the culture in artificial seawater with galactose and monosodium glutamate, pH at 7.2–7.5, and temperature at 35 ± 0.5 °C exhibits pigment production with the yield of 0.700–0.800 g/L. The characterization studies revealed that the pigment is orange reddish pink in color, soluble in water, insoluble in both polar and non-polar organic solvents, and decolorized when exposed to H2O2, KMnO4, and K2Cr2O7. UV-visible spectrum exhibits maximum absorption peak at 330 nm and mild shoulders at 400 and 500 nm. The pigment displayed both antioxidant and reducing power properties when exposed with ABTS and ferricyanide, respectively. The sun protection factor of the pigment was determined as 9.9. The cytocompatibility of the obtained pigment studied using HaCaT cells revealed that the cells are viable up to 500 μM. Experiments on UVB protection of the pigment using HaCat cells demonstrated the appreciable protective effect by reducing the ROS generation upon UVB exposure. In conclusion, the pigment obtained from the marine imperfect fungus showed structural similarity with DHICA which is confirmed by LC-MS. The presence of metal ions in the growth medium mediates the reaction to proceed towards DHICA formation. The SPF analysis, antioxidant property, and the UVB protection studies authenticate the potential use of pigment for skin care.

中文翻译:

探索从海洋不完全真菌中提取的黑色素前体对UVB的保护功效:具有体外条件下的表征和应用研究。

本研究探索了从海洋上不完美的非孢子形成构巢曲霉(Aspergillus nidulans sp)潜在稳定的黑色素前体的生产,表征和应用SG 28菌株。在半乳糖和谷氨酸钠的人工海水中培养物的生长,pH在7.2–7.5,温度在35±0.5°C时,色素生成量为0.700–0.800 g / L。表征研究表明,该颜料为橙红色粉红色,可溶于水,不溶于极性和非极性有机溶剂,并且在暴露于H 2 O 2,KMnO 4和K 2 Cr 2 O 7时会脱色。。紫外可见光谱在330 nm处显示最大吸收峰,在400和500 nm处显示温和的肩峰。当分别与ABTS和铁氰化物接触时,该颜料既显示抗氧化剂性能,又显示还原力性能。颜料的防晒系数确定为9.9。使用HaCaT细胞研究的所得色素的细胞相容性表明,该细胞在高达500μM的条件下仍具有活力。使用HaCat细胞对颜料进行UVB保护的实验通过减少UVB暴露时的ROS产生了明显的保护作用。总之,从海洋不完全真菌获得的色素显示出与DHICA的结构相似性,这已通过LC-MS证实。生长培养基中金属离子的存在介导了反应,以进行DHICA的形成。SPF分析,抗氧化性能,
更新日期:2018-05-15
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