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Cytogenetic instability of chromosomal nucleolar organizer regions (NORs) in cloned mouse L929 fibroblasts.
Chromosome Research ( IF 2.6 ) Pub Date : 2019-01-04 , DOI: 10.1007/s10577-018-9598-8
Olga V Zatsepina 1
Affiliation  

Ribosomal DNA (rDNA) gene codes for 18S, 5.8S, and 28S rRNA form tandem repetitive clusters, which occupy distinct chromosomal loci called nucleolar organizer regions (NORs). The number and position of NORs on chromosomes are genetic characteristics of the species although within a cell, the NOR sizes can significantly vary due to loss or multiplication of rDNA copies. In the current study, we used mouse L929 fibroblasts, the aneuploid cells which differ in the FISH- and Ag-NOR numbers, to examine whether the parental NOR variability is inherited in clones. By statistical analysis, we showed that the cloned fibroblasts were able to restore the NOR numerical characteristics of the parental cells after long-term culturing. These results support the idea that mammalian cells may have mechanisms which control the number and activity of NORs at the population level. In L929 fibroblasts, we also regularly observed laterally asymmetry of FISH-NORs that evidenced in an unequal distribution of the mother rDNA copies between the daughter cells in mitosis.

中文翻译:

克隆的小鼠L929成纤维细胞中染色体核仁组织者区域(NORs)的细胞遗传学不稳定性。

核糖体DNA(rDNA)基因编码18S,5.8S和28S rRNA形成串联重复簇,它们占据了称为核仁组织区(NORs)的独特染色体位点。NOR在染色体上的数量和位置是物种的遗传特征,尽管在细胞内,NOR的大小可能会由于rDNA拷贝的丢失或繁殖而显着变化。在当前的研究中,我们使用小鼠L929成纤维细胞(FISH和Ag-NOR数不同的非整倍体细胞)来检查亲本NOR变异是否在克隆中遗传。通过统计分析,我们表明,克隆的成纤维细胞能够在长期培养后恢复亲本细胞的NOR数值特征。这些结果支持了哺乳动物细胞可能具有在群体水平上控制NOR的数量和活性的机制的想法。在L929成纤维细胞中,我们还定期观察到FISH-NOR的横向不对称现象,这在有丝分裂的子细胞之间母rDNA拷贝的不均等分布中得到了证明。
更新日期:2019-11-01
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