Macrophages are crucial to host defense, functioning in innate and cell-mediated immunity. MicroRNAs (miRNAs) are small non-coding RNA molecules that repress transcription and protein production. Little is known about miRNA expression in primary human macrophages, or about how macrophage miRNAs contribute to both normal macrophage function and to the pathogenesis of disease in humans. Using Western blot analyses, we demonstrated the production of miRNA machinery proteins by human primary macrophages. Using two different miRNA array techniques, we identified 119 miRNAs expressed by human primary macrophages, including hsa-let-7a, miR-16, -23a, 30b, -103, -146a, -212, and -378 and validated them by quantitative RT-PCR. Our findings provide a knowledge base to which macrophage miRNA expression in organ-specific macrophages or disease processes may be compared in humans.

中文翻译：

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MicroRNA 由人类原代巨噬细胞表达和加工。

巨噬细胞对宿主防御至关重要，在先天性和细胞介导的免疫中发挥作用。MicroRNAs (miRNAs) 是小的非编码 RNA 分子，可抑制转录和蛋白质的产生。关于 miRNA 在原代人类巨噬细胞中的表达，或者巨噬细胞 miRNA 如何促进正常的巨噬细胞功能和人类疾病的发病机制，我们知之甚少。使用蛋白质印迹分析，我们证明了人类原代巨噬细胞产生 miRNA 机械蛋白。使用两种不同的 miRNA 阵列技术，我们鉴定了人类原代巨噬细胞表达的 119 种 miRNA，包括 hsa-let-7a、miR-16、-23a、30b、-103、-146a、-212 和 -378，并通过定量验证它们逆转录聚合酶链反应。