Abstract In recent years there has been much interest in development of multifunctional drug delivery systems. In this work, liposomes that contain doxorubicin (Dox), a potent anticancer drug, and graphene nanosheets (GNS) were prepared. The GNSs have excellent optical properties, such as photoluminescence which enables tracking of the liposomes, high absorption in ultra violet region of electromagnetic spectrum which can be exploited in photodynamic and photothermal therapy, and low toxicity to mammalian cells. Nanoliposomes were prepared using the thin film hydration method. Dox and GNSs were loaded to the liposomes during the hydration of the lipid film. Liposomes were characterized and the profile of in vitro drug release, cellular uptake, and cytotoxicity of the prepared liposomes on MCF-7 cells were determined. Despite the earlier reports, the liposomes have kept their spherical structures in the presence of GNSs. The cytotoxicity of liposomal Dox and GNSs were shown to be higher than the free forms of them. Novel nanoliposomes that contain GNSs have provided a multi-functional system with the potential of tracking, photodynamic and photothermal therapy. Further improvements of this versatile nanosystem would be promising for treatment of cancer.

中文翻译：

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脂质体阿霉素-石墨烯纳米片的制备及其体外抗癌作用评价

摘要 近年来，人们对多功能给药系统的开发产生了浓厚的兴趣。在这项工作中，制备了含有多柔比星 (Dox)、一种强效抗癌药物和石墨烯纳米片 (GNS) 的脂质体。GNS 具有优异的光学特性，例如能够跟踪脂质体的光致发光、可用于光动力和光热疗法的电磁光谱紫外线区域的高吸收以及对哺乳动物细胞的低毒性。使用薄膜水合法制备纳米脂质体。在脂质膜的水合过程中，Dox 和 GNS 被加载到脂质体中。对脂质体进行表征，并确定制备的脂质体对 MCF-7 细胞的体外药物释放、细胞摄取和细胞毒性的特征。尽管有早先的报道，在 GNS 存在的情况下，脂质体保持其球形结构。脂质体 Dox 和 GNS 的细胞毒性显示高于它们的游离形式。含有 GNS 的新型纳米脂质体提供了一个多功能系统，具有跟踪、光动力和光热疗法的潜力。这种多功能纳米系统的进一步改进将有望用于治疗癌症。