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A role of age-dependent DNA methylation reprogramming in regulating the regeneration capacity of Boea hygrometrica leaves.
Functional & Integrative Genomics ( IF 2.9 ) Pub Date : 2019-08-14 , DOI: 10.1007/s10142-019-00701-3
Run-Ze Sun 1 , En-Hui Zuo 1 , Jin-Feng Qi 1 , Yang Liu 1 , Chih-Ta Lin 1 , Xin Deng 1
Affiliation  

Plants can regenerate new individuals under appropriate culture conditions. Although the molecular basis of shoot regeneration has steadily been unraveled, the role of age-dependent DNA methylation status in the regulation of explant regeneration remains practically unknown. Here, we established an effective auxin/cytokinin-induced shoot regeneration system for the resurrection plant Boea hygrometrica via direct organogenesis and observed that regeneration was postponed with increasing age of donor plants. Global transcriptome analysis revealed significant upregulation of genes required for hormone signaling and phenylpropanoid biosynthesis and downregulation of photosynthetic genes during regeneration. Transcriptional changes in the positive/negative regulators and cell wall-related proteins involved in plant regeneration, such as ELONGATED HYPOCOTYL5 (HY5), LATERAL ORGAN BOUNDARIES DOMAIN, SHOOT-MERISTEMLESS, and WUSCHEL, were associated with the regeneration process. Comparison of DNA methylation profiling between leaves from young seedlings (YL) and mature plants (ML) revealed increased asymmetrical methylation in ML, which was predominantly distributed in promoter regions of genes, such as HY5 and a member of ABA-responsive element (ABRE) binding protein/ABRE binding factor, as well as genes encoding glycine-rich cell wall structural protein, CENTRORADIALIS-like protein, and beta-glucosidase 40-like essential for shoot meristem and cell wall architecture. Their opposite transcription response in ML explants during regeneration compared with those from YL demonstrated the putative involvement of DNA methylation in regeneration. Moreover, a significant lower expression of DNA glycosylase-lyase required for DNA demethylation in ML was coincident with its postponed regeneration compared with those in YL. Taken together, our results suggest a role of promoter demethylation in B. hygrometrica regeneration.

中文翻译:

年龄依赖性DNA甲基化重编程在调节潮水两叶的再生能力中的作用。

植物可以在适当的培养条件下再生新个体。尽管已经逐步阐明了芽再生的分子基础,但是年龄依赖的DNA甲基化状态在调节外植体再生中的作用实际上仍是未知的。在这里,我们为复活植物Boea hygrometrica建立了有效的生长素/细胞分裂素诱导的芽再生系统通过直接器官发生,并观察到再生随着供体植物年龄的增加而推迟。全局转录组分析显示,激素信号转导和苯丙烷类生物合成所需的基因显着上调,而再生期间光合基因的下调。参与植物再生的正/负调节剂和细胞壁相关蛋白的转录变化,例如长绒毛Hypocotyl5(HY5),横向器官边界域,短生无性和WUSCHEL,与再生过程相关。比较幼苗(YL)和成熟植物(ML)叶片之间的DNA甲基化分布,发现ML中不对称甲基化增加,这主要分布在HY5等基因的启动子区域以及ABA反应元件(ABRE)结合蛋白/ ABRE结合因子的成员,以及编码富含甘氨酸的细胞壁结构蛋白,CENTRORADIALIS-like蛋白和β-葡萄糖苷酶40-like的基因,是分生组织和细胞壁必不可少的建筑。与来自YL的外植体相比,它们在ML外植体中的相反转录响应与YL的反应表明,DNA甲基化可能参与了再生。此外,与YL中相比,ML中DNA去甲基化所需的DNA糖基化酶裂解酶的显着较低表达与其延迟的再生相吻合。两者合计,我们的结果表明启动子去甲基化在B.hygrometrica再生中的作用。
更新日期:2019-08-14
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